International Journal of Horticulture 2015, Vol.5, No.3, 1
-
12
5
Extraction and analysis of pendimethalin
Pendimethalin was extracted from the soil and
rhizome samples with
methanol and the extract was
filtered and evaporated at 60°C to about 10 ml. The
concentrated extract was transferred to separatory
funnel and 30 ml of 5 % aqueous NaCl was added.
The contents were partitioned with n-hexane passed
through anhydrous sodium sulphate. Hexane layer was
concentrated on rotary vacuum evaporator at 60°C to
approximately 5 ml and quantified by Gas
Chromatograph (GC-Chemito Model 8610) equipped
with
63
Ni electron capture detector.
Instrumental conditions
Detector
Electron capture detector
Column
Megabore capillary column D13-30 m
×
0.53 mm ID-BPL 0.5 µm
Injector
240
o
C
Column
185-220
o
C
Detector
260
o
C
Carrier gas flow rate
40 ml min
-1
Retention time
4.82 min
Extraction and analysis of atrazine
Homogenized soil and rhizome samples of 10 g were
taken and extracted with 10 ml of acetonitrile-water
(90/10 v/v) by shaking for 35 minutes in a 500 ml flask
in mechanical shaker. Extract was filtered by gravity and
washed with an additional 25 ml acetonitrile-water. After
that 300 ml of water was added and the solution was
extracted with 3 x 25 ml portion of dichloromethane by
shaking for 5 minutes each time. These extracts were
combined and evaporated to dryness. The residue was
re-dissolved in an appropriate volume (2 ml) of hexane
phase for HPLC analysis.
[min.]
Time
2
4
6
8
10
[mV]
Voltage
-100
0
100
200
300
Pendimethalin
C:\IRIS32\WORK1\DATA\2013\TURMERIC\PENDIMETHALINSTD0.5 PPM
4.820
