International Journal of Horticulture 2015, Vol.5, No.3, 1
-
12
7
Instrumental conditions
Detector
Electron capture detector
Column
Megabore capillary column D13-30 m
×
0.53 mm ID-BPL0.5 µm
Injector
240
o
C
Column
185-190
o
C
Detector
260
o
C
Carrier gas flow rate
40 ml min
-1
Retention time
2.51 min
Extraction and analysis of metsulfuron methyl
50 g of fine soil and rhizome sample were transferred to
250 ml conical flask and extracted with 100 ml of
acetonitrile and 250 ml of Buffer-B. The flask was
stoppered and shaken for 2 hr in a reciprocating shaker
and kept aside for flocculation of the soil particles. The
supernatant was filtered under vacuum through a
Whatman No.1 filter paper using 2 ×50 ml acetonitrile
as washing solvent. The filtrate thus obtained was
concentrated for cleanup. The concentrated extract was
transferred to 500 ml separatory funnel with 20 ml of
Buffer-A and was partitioned with methylene chloride
(3 × 50 ml). The methylene chloride layer was
discarded and the aqueous layer was taken in a 250 ml
beaker for adjusting the pH to 3.5 using 10 % HCl. It
was transferred into a 250 ml separatory funnel and
extracted with 3 × 50 ml of toluene. After the
separation of layers, the aqueous layer was discarded
and the organic layer was filtered through anhydrous
sodium sulphate. The combined organic layer was
evaporated in a rotary vacuum evaporator and
injection into HPLC.
[min.]
Time
2
4
6
8
10
[mV]
Voltage
-100
-50
0
50
100
Oxadiargyl
C:\IRIS32\WORK1\DATA\FEB2014\0.5 PPM OXYDIARGYL
2.510
