IJH-2017v7n9 - page 15

International Journal of Horticulture, 2017, Vol.7, No. 9, 64-75
74
The severity of damage and weight loss of the protected cowpea was done by counting the number of seeds with
hole and weighing them after no emergence of adult was observed. The percentage seed damage and weight loss
were calculated using the formulae below:
5.5 Statistical analysis
Data obtained were subjected to One-Way analysis of variance and means were separated with Duncan’s Multiple
Range Test. Also, data obtained from mortality were subjected to Probit analysis to calculate the lethal
concentration required to achieve 50 and 95% mortality within 72 h (LC
50
and LC
95
). General Linear Model
(GLM) was used to determine the interactive effect of the plant part used, extraction methods and concentrations.
Linear regression analysis was done to determine interaction between adult mortality and oviposition as well as
between adult emergence and weight loss.
Authors’ contributions
Both authors designed and carryout the research, author 2 carryout the statistical analysis while the two authors wrote the paper.
Refrences
Abd-Elhady 2012, Insecticidal activity and chemical composition of essential oil from Artemisia judaica L. against Callosobruchus maculatus (f.) (Coleoptera:
Bruchidae). Journal of Plant Protection Research, 52(3):347-352. DOI: 10.2478/v10045-012-0057-9
Akinneye J.O., and Ogungbite O.C., 2013, Insecticidal activities of some medicinal plants against Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae)
on stored maize, Arch Phytopathol. Plt Protect., 46:(10):1206-1213
Akinneye J.O., and Ogungbite O.C., 2016, Entomotoxicant potential of some medicinal plant against Ephestia cautella infesting cocoa bean in storage. Intl J
Appl Sci and Eng, 14(1): 59-68
Al-Qahtani A.M., Al-Dhafar Z.M., and Rady M.H., 2012, Insecticidal and biochemical e
ff
ect of some dried plants against Oryzaephilus surinamensis
(Coleoptera-Silvanidae). The J Basic Appl Zoo, 65: 88
93
Ashamo M.O., and Ogungbite O.C., 2014, Extracts of medicinal plants as entomocide against Sitotroga cerealella (Olivier) infestation on paddy rice, Medicinal
Plant Research, 4(18): 1-7
Ashamo M.O., Odeyemi O.O., and Ogungbite O.C., 2013, Protection of cowpea, Vigna unguiculata L. (Walp.) with Newbouldia laevis (Seem.) extracts against
infestation by Callosobruchus maculatus (Fabricius). Arch Phytopatho Plt Protect, 46(11): 1295-1306
Berenbaum M.R., and Zangerl A.R., 1996, Phytochemical diversity: adaptation or random variation? Recent Adv Phytochem (in press)
Doughari J.H., 2012, Phytochemical: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents, Phytochemical - A
Global Perspective of Their Role in Nutrition and Health, Dr Venketeshwer Rao (Ed.), ISBN:978-953-51-02960
Epidi T.T., and Udo I.O., 2009, Biological Activity of Ethanolic Extract Fractions of Dracaena arborea Against Infestation of Stored Grains by Two Storage
Insect Pests. Pakistan J. Bio Sci., 12(13): 976-980. DOI: 10.3923/pjbs.2009.976.980
Fazali F., Zulkhairi A., Nurhaizan M.E., Kamal N.H., Zamree M.S., and Shahidan M.A., 2011, Phytochemical screening, in vitro and in vivo antioxidant
activities of aqueous extract of Anacardium occidentale Linn. and its effects on endogenous antioxidant enzymes in hypercholesterolemic induced rabbits.
Research Journal of Biological Sciences, 6 (2): 69-74
Forim M.R., Da-silva M.F.G.F., and Fernandes J.B., 2012, Secondary metabolism as a measurement of efficacy of botanical extracts: The use of Azadirachta
indica (Neem) as a model. In: Perveen F (Ed.), Insecticides-Advances in Integrated Pest Management, 2012, p367-390
Hollingworth R., Ahmmadsahib K., Gedelhak G., and McLaughlin J., 2010, New inhibitors of complex I of the mitochondrial electron transport chain with
activity as pesticides. Biochemical Society and Transgenesis, 1994; 22, 230
33
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