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achieved on this medium (Figure 2) followed by TM4 whereas TM1, TM3, TM7, TM8 and TM9 all showed poor
response for
in vitro
tuberization (Table 3).
Table 2 Analysis of variance (ANOVA) table: ANOVA depicts the significant variation in response of photomixotrophic conditions
of tuberization media on in vitro tuberization
Source
Df
SS
MSS
F
P
Media
8
22661.1
2832.63
822**
0.00
Error
18
62.0
3.44
Total
26
22723.1
Note: ** = Highly significant (P value <0.01)
Table 3 All-pairwise comparisons test of no. of microtubers from tuberization media to be studied
Media
TM 5
TM 4
TM 2
TM 6
TM 1
TM 3
TM 7
TM 8
TM 9
Mean
81
a
60
b
37
c
25
d
0.1
e
0.1
e
0.1
e
0.1
e
0.1
e
Note: α = 0.05; SE= 1.5154
Figure 1 A graphical representation of response of tuberization media, on average number of microtubers per explant
Figure 2 Response of photomixotrophic conditions of TM5 on
in vitro
tuberization
Effect of light and dark regimes on in vitro microtuberization
: In this study effect of light and dark regimes were
also investigated. For this purpose, cultured explants were kept in complete dark regime as well as in 16/8 hour
light/dark regime. When explants were maintained in dark regime at 21± 1
0
C, then microtubers were induced.
