International Journal of Horticulture, 2017, Vol.7, No.17, 138-145
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But the case was otherwise in case when explants were incubated at 26 ± 1
0
C under 16/8 hrs light/dark regimes.
In this case, instead of microtubers formation, flowering was induced (Figure 3). In this study, transitions of
cultured material from dark to light condition and vice versa were also done to examine the effect. Thus
microtubers produced in dark conditions were when kept in light conditions for some time; they turned to
greenish black in color (Figure 4). This might be due to the synthesis of chlorophyll. Similarly, when the cultured
material that was initially kept in light/dark regime and they showed flowering, when kept in dark conditions they
showed response towards microtuberization (Figure 5).
Figure 3 Responses of in
vitro
microtuberization under 16/8 hrs light/darkregime
Figure 4 Response after transferring culture tubes from dark to light regimes
Figure 5 Response after transferring culture tubes from light to dark regimes
Effect of photomixotrophic conditions on morphological parameters
: The responses of all tuberization media
were also different regarding days to stolon induction, days to tuber initiation/induction, days to tuber maturation
and stolon length (Table 4). Microtuber maturation data were recorded on the basis skin color. When skin color
