International Journal of Horticulture, 2017, Vol.7, No.17, 138-145
138
Research Article
Open Access
Evaluating the Effect of Photomixotrophic Conditions for Expedite and
Efficient
In Vitro
Tuberization System on the Morphological, Biochemical,
Anatomical and Physiological Characteristics of Microtubers
Siddra Ijaz
1
, Erum Shah
2
, and Imran-ul-Haq
3
1 Centre of Agricultural Biochemistry and Biotechnology/US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture
Faisalabad, Pakistan
2 Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
3 Department of Plant Pathology, University of Agriculture Faisalabad, Pakistan
Corresponding email:
International Journal of Horticulture, 2017, Vol. 7, No. 17 doi:
Received: 07 Jun., 2017
Accepted: 30 Jun., 2017
Published: 20 Jul., 2017
Copyright
©2017 Ijaz et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Ijaz S., Shah E, and Imran-ul-Haq, 2017
,
Evaluating the effect of photomixotrophic conditions for expedite and efficient in vitro tuberization system on the
morphological, biochemical, anatomical and physiological characteristics of microtubers
,
International Journal of Horticulture, 7(17): 138-145 (doi:
)
Abstract
In vitro
tuberization is becoming a swift approach to expedite seed tuber production and multiplication for various
purposes such as germplasm conservation as well as exchange, experimentation in basic research,
in vitro
selection of desirable
agronomic traits and disease free tuber production. Therefore, this study was designed for optimizing
in vitro
tuberization system
using different photomixotrophic conditions in tissue culture regime. For this purpose, sucrose, BAP and kinetin were used at
different levels and combinations for evaluating their effects on proficient and expedite
in vitro
tuberization system in selected local
potato cultivars, PRI-Red. The results showed that tuberization medium, TM5 (100 g/l sucrose; 4.75 mg/l kinetin but deprived of
BAP) proved to be best in terms of maximum number of microtubers per explant achieved. Subsequently, morphological,
anatomical, physiological and biochemical characteristics of these
in vitro
grown microtubers were studied for evaluating the effects
of photomixotrophic conditions on the said characteristics of microtubers.
Keywords
Potato; Tissue culture; Potato anatomy; Proximate analysis; Photomixotrophic condition
1 Introduction
Potato is the fourth most important food crop, after wheat, rice and maize. This crop is grown under a large area
all over the world and is being used as a staple food in developing countries like Pakistan, Bangladesh and India.
In our agro-climatic conditions, this crop is generally grown vegetatively through tubers. For vegetative
reproduction, tuber is used as a seed.Whereas in conventional breeding system, crosses are made that are
followed by the selection and screening of subsequent progenies possessing desired traits. These selected F1
material is then clonally or vegetatively propagated that result in the fixing of these required traits (Sleper and
Poehlman, 2006). However the F1 heterozygous material gives heterotic effect results in hybrid vigor while on
selfing it leads to inbreeding depression (Arndt and Peloquin, 1990). Hence, in conventional seed potato
production the risk of viral, bacterial and fungal diseases is increased with every round of progeny multiplication
in the field.
The total cost on potato seed is very high, because just cost on seed transportation and storage is about 47% of the
total cost. Though best quality potato seed is generally imported from other countries that results in huge
economic burden. Therefore in this crop, different strategies are being adopted for seed production either via
conventional means or non-conventionally. Among these various strategies and techniques for seed production in
potato,
in vitro
microtuberization holds numerous intrinsic worth in terms of ease in transportation, handling and
long term storage. Whereas potato tuberization is very complex developmental process which is regulated by
many factors (Altindal and Karadogan, 2010). Tuber growth and developmental processes are very difficult to
study in field grown plants because of obscuring effect of soil and little synchronization of the tuberization
