International Journal of Horticulture, 2017, Vol.7, No. 5, 33-39
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Cluster II
Cluster II contained 18 genotypes; GA-2002, Lasani-2008, 98FJ13, 9870, Noshera-96, WC-20, WC-24, WC-4,
WC-13, WC-26, Baktawar-93, Sarsabz, Sahar-2006, Pasban-90, NARC-2009, Kohistan-97, Mehran-89 and
Tatara. This cluster constitutes about 60 percent of the total genotypes used in the study. GA-2000 and Tatara are
the outliers. At the linkage distance of 0.30 this cluster contains three sub-clusters. Sub-cluster 1 has ten genotypes;
GA-2002, Lasani-2008, 98FJ13, 9870, Noshera-96, WC-20, WC-24, WC-4 and WC-13. These genotypes showed
close affiliation with each other with less variability. Sub-cluster 2 comprises of three genotypes WC-26,
Baktawar-93, Sarsabz and Sahar-2006. Theses genotypes have association with the sub-cluster 1 but
disassociation with sub-cluster 3, which constituted about five genotypes; Pasban-90, NARC-2009, Kohistan-97,
Mehran-89 and Tatara. Tatara is an outlier of the main cluster II. All the genotypes included in cluster II have
close association with each other but have a divergence as compare to cluster I and cluster III. Hence these
genotypes can be put under hybridization program for the future breeding strategies.
Figure 4 Dendrogram representing the genetic diversity on the basis of polypeptide banding pattern among 30 bread wheat genotypes
Cluster III
Figure 2 showed three genotypes; 03FJ-26, Pirsabak-91 and 8973 which constituted about 10 percent of the total
genotypes used in the study. Advanced breeding line 8973 is an outlier in this cluster and overall genotypes under
investigation. This can be concluded that 8973 is a much diverse genotype and showed great genetic variability.
Kakaei et al. (2011) done the cluster analysis with UPGMA method separated the genotypes into 2 clusters with
banding pattern and fragment size ranged from 78, 70, 54, 35, 23, 24 and 16 kDa. Our findings are not very much
similar to these results, fragment size of 150 KDa and above were isolated in our study. The studies of Yoon et al.
(2010) showed the protein fragment sizes range from 160 to 22 KDa, these finding are very much in accordance
with our findings. Alnaddaf et al. (2011) also used SDS-PAGE for the quality assessment protein fragments for
the detection of suitable breeding line for the future breeding program. Ahmed et al. (2010) assessed genetic
diversity among 32 advanced breeding lines wheat using biochemical markers (SDS-PAGE) and concluded higher
molecular weight subunits (120 KDa) showed immense polymorphism than the lesser molecular weight. These
results are not in association with our findings because in our findings lowers molecular weight protein fragments
generated much polymorphic banding patterns i.e; 60 KDa to 20 KDa. Ayed et al. (2010) assessed genetic
variability by using seed storage-proteins through sodium dodecylsulphate polyacrylamide gel electrophoresis
