International Journal of Aquaculture, 2017, Vol.7, No.23, 143-158
151
Figure 1 OD value of the selected gut microbe isolated from
O. niloticus
(a) ONF1P (b) ONF7.1C grown in mucus of
O. niloticus
incubated for 24 h, 48 h and 72 h duration in mucus
After 21 days of small-scale
in vivo
experiment, it was noticed that along with the control set intra-peritoneal
injection of the isolates (ONF1P and ONF7.1C) did not induce any pathological signs/disease symptoms or
mortalities in both treatment groups.
Based on the nucleotide homology and phylogenetic analysis of the 16S rRNA partial gene sequences by
nucleotide blast in the National Centre for Biotechnology Information (NCBI) GenBank and RDP databases,
strain ONF1P was identified as
Bacillus sonorensis
(Accession No. KT362744). On the other hand, based on the
nucleotide homology and phylogenetic analysis of the 18S rRNA partial gene sequence by nucleotide blast in the
NCBI GenBank, strain ONF7.1C was identified as
Pichia kudriavzevii
(Accession No. KT582009). Phylogenetic
relationships of the identified bacteria (Figure 2) and yeast (Figure 3) isolates with other closely-related strains
retrieved from NCBI GenBank are presented in the dendogram.
Figure 2 Dendrogram showing phylogenetic relations of the bacterial strain,
Bacillus sonorensis
ONF1P, with other closely related
strains retrieved from NCBI GenBank and RDP
0
0.2
0.4
0.6
0.8
1
1.2
1.4
24
48
72
ONF1P
ONF7.1C
OD at 600nm
Incubation period (hr)
