Genomics and Applied Biology 2018, Vol.9, No.2, 6-13
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And the optimal concentration of GA3 for seeds was 100 mg/L, because GA3 had induction for amylase, which
could be stimulants of cell division. And the required concentration of the roots growth was different from the
germination. The whole seed of
Sophora davidii
could absorb water, but the main part was hilum, the raphe was
weak. This research provided a theoretical basis for the germination mechanism of
Sophora davidii
seeds, and it
also could help the cultivation of
Sophora davidii
.
3 Materials and Methods
3.1 Experimental materials
The seeds of
Sophora davidii
picked from College of Agriculture, Guizhou University on October 20
th
2015
(Landmark: 26°25'39.62"N, 106°40'5.81"E).
3.2 Experimental reagents
GA3, starch, maltose, citric acid, sodium citrate, 3, 5-dinitrosalicylic acid, sodium hydroxide and seignette salt
were taken from the laboratory of College of Agriculture, Guizhou University.
3.3 The preparation of reagents
Prepared the following solutions: (1) 1000 ml mother solution of GA3 with the concentration of 1000 mg/l. (2)
500 ml citric acid buffer solution with PH5.6 and the concentration of 0.1 mol/l. (3) 1000 ml 3, 5-dinitrosalicylic
acid preserved in a dark place, sealed and refrigerated. (4) 100 ml maltose standard solution with the concentration
of 1 mg/ml. (5) 100 ml NaOH with the concentration of 0.4 mol/l. (6) 500 ml starch solution with the
concentration of 1%.
3.4 The treatment of seeds
This research took 300 seeds from provided seeds randomly, polished them by gauze until the scratches on them.
And put them into constant temperature oven (25°C) with warm water of 25°C. After 36 h, the seeds should be
cleaned and divided into 6 groups randomly, 50 seeds in each groups(Li, 2004); We took another 300 seeds
without polishing, and put them into constant temperature oven (25°C) with warm water of 25°C. After 36 h, the
seeds would be cleaned and divided into 6 groups randomly, 50 seeds in each groups.
3.5 The treatment methods of GA3
This research grouped the soaked seeds according to Table 3: (1) the group treated with GA3 after polishing; (2)
the group treated with GA3 without polishing; (3) The group treated with sealing after polishing. Using solutions
from 5 kinds of concentration gradients as culture solution for (1) and (2) respectively under the same conditions,
cultivated them in 5 culture dishes, which had multi-layer gauze, set control group, and put them in constant
temperature oven (25°C); (3) treated by sealing, and then cultivated it in culture dish with gauze and took distill
water as culture solution. Then set a control group without sealing, and put it in constant temperature oven (25°C).
Table 3 Different GA concentration gradient settings and sealing treatments on different parts of
Sophora davidii
Handling method
Concentration gradient and sealing parts
1
2
3
4
5
6
Grinding+GA3
50mg/L
100mg/L 200mg/L
300mg/L
500mg/L Distilled water
Not polished+GA3
50mg/L
100mg/L 200mg/L
300mg/L
500mg/L Distilled water
Vaseline seal processing
Hilum
Raphe
Hilum and raphe Except hilum and raphe Not sealed
3.6 The physiological parameters monitoring of
Sophora davidii
seeds
In order to monitoring the physiological parameters of
Sophora davidii
seeds, this research used methods of
weighing average quality of seeds, measuring the length, width and thickness, shooting the morphology of dry
seed, measuring the thickness of seed coats, etc. by sensitive balance, vernier caliper, 01ympus SZX12 anatomical
lens and the App of Image-Proplus.
