Genomics and Applied Biology 2018, Vol.9, No.2, 6-13
10
Figure 6 Effect of Vaseline sealing treatment on root length of
Sophora davidii
seedlings
Table 2 Effect of GA3 on the activity of
-amylase in
Sophora davidii
Concentration of GA
(mg/l)
Alpha amylase activity
[mg/(g.min)]
0 d
3 d
6 d
9 d
0
0.123±0.0013
0.322±0.0121
0.931±0.0865
0.873±0.0023
50
0.122±0.0015
0.707±0.0171
1.045±0.0672
0.988±0.0064
100
0.123±0.0032
0.76±0.0098
1.619±0.0522
0.873±0.0036
200
0.122±0.0024
1.02±0.0018
2.135±0.0171
1.045±0.0015
300
0.121±0.0061
0.727±0.0028
1.603±0.0432
0.931±0.0024
500
0.122±0.0052
0.687±0.0971
1.131±0.007
0.971±0.0024
2 Discussion
The hard seed of
Sophora davidii
belongs to the seed coat restriction, and the seed coat is very tough, which
hindered the release of CO
2
and the entry of O
2
, thus inhibited the seed respiration, It could not guarantee the
enough energy supplied for the germination of seeds, and constrained the embryo. The results of this research
confirmed the theoretical characteristics of
Sophora davidii
: smaller volume and quality, smaller water-absorption
area, but it had thicker seed coats, during the process of germination and imbibition, embryo absorption and gas
exchange would be impeded and limited, thus decreased the germination rate of seeds.
Without any treatment, the germination rate of
Sophora davidii
seeds is extremely low. And it could not accurately
reflect the actual germination ability of the seed, and could not obtain greater economic benefit in actual
production. This research found that the mechanically abrading promoted the germination of seeds, the
germination rate could reach 94% on 10
th
day, but the activity of
-amylase was weak and the germination rate
was slow. From the 5
th
day to the 10
th
day, the optimal GA3 concentration of the seeds germination was 100 mg/L,
but treated by optimal concentration of GA3, the germination rate of seeds on the 10
th
was still 1.90%. It was an
abnormal condition, and the reason for it was that the seeds were unfinished, the culture medium was not absorbed
in time, the concentration of culture medium increased and the optimal concentration moved forward over time.
The seeds treated by GA3 up to the maximum of germination on the 6
th
day, which showed that the GA3 had a
positive effect on the seed germination, could improve the activity of amylase in seeds, promote seed germination
in advance, and it also could shorten the germination cycle.
Under the conditions of polishing treatment, the optimal concentration of GA3 for seeds was 200 mg/L, in case of
treatment without polishing, the optimal concentration of GA3 for seeds was 100 mg/L. When the seeds treated by
GA3 with 200 mg/L concentration after polishing, it had the strongest activity of amylase, the fast germination
and the higher germination rate. GA3 improved the growth of roots after the germination of
Sophora davidii
seeds.
