Genomics and Applied Biology 2018, Vol.9, No.2, 6-13
12
3.7 The measurement of
-amylase activity
By 3,5-dinitrosalicylic acid method, this research measured the
-amylase activity of
Sophora davidii
seeds,
which treated by GA with polishing on the 1
st
day, 3
rd
day, 6
th
day and 9
th
day respectively, measured the activity
of seeds indirectly (Wang, 2006).
-amylase activity (mg/g·min)=
(
( )
( )
)
(
( )
( )
( )
(Hu and Niu, 2009).
3.8 The measurement of the seeds germination rate
The seeds germination rate of (1), (2), (3) were measured respectively:
Germination rate (%) =
100%
3.9 The measurement of the root growth of seeds
The each concentration and the growth of roots treated in (1), (2), (3) were measured respectively, the results were
showed by scatter diagram.
3.10 The treatment of experimental data
This experiment used Image-Proplus, Excel and SPASS20.0 to handle and analyze statistical data, each
experiment repeated at least 3 times.
Authors’ contributions
LJ was the executor of the experimental design and research. XR completed the data analysis and LC completed the first draft of this
thesis. ZYF took part in the experiments, and YY was the designer and principal of this project. TXX directed the design of
experiment, and SW was in charge of the writing and revising this thesis. All the authors read and approved the final manuscript.
Acknowledgments
This study was funded by the Science and technology projects in Guizhou (No. LH [2016] 7210), the key laboratory construction
projects of the regulation of plant physiology and development in Guizhou, the key laboratory construction projects in Guizhou (No.
z [2011] 4005), the innovation team in education ministries of biodiversity protection and sustainable utilization in karst mountain,
the program for Changjiang Scholars and Innovative Research Team (IRT1227) and the program of doctor research start-up in
Guizhou Normal University (11904-0514012).
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