| Research Article | 4 |
| Abstract | 4 |
| Keywords | 4 |
| Abbreviation | 4 |
| Introduction | 4 |
| 1 Material and Method | 6 |
| 1.1 Collection of Samples | 6 |
| 1.2 Contamination Prevention of Fossil Human Bones | 6 |
| 1.3 Decalcifications of Bone and Genomic DNA Extraction | 6 |
| 1.4 Digestion of Fossil DNA by Restriction Endonucleases | 8 |
| 1.5 Restriction digestion of DNA protocols | 8 |
| 1.6 Polymerase Chain Reaction (PCR) Amplification of Sex Determination | 8 |
| 1.7 Gel Electrophoresis of PCR Amplicons | 9 |
| 1.8 Evaluation of Y Chromosome Lineage PCR-RFLP Analysis | 10 |
| 1.9 Microsatellite DNA Profiling and Authentication | 10 |
| 1.10 Statistical Analyses | 10 |
| 2 Results and Discussion | 10 |
| 3 Concluding Remarks | 11 |
| Acknowledgment | 12 |
| References | 13 |