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Genomics and Applied Biology 2014, Vol. 5, No. 4, 1-5
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3
complex which may also be the cause of the reduced
chlorophyll content.
2.5 Carotenoids (mg/g)
The result on Carotenoid content was significantly
differed in all the treatments. Among the treatments,
T
1
showed highest Carotenoid content in green gram
both CO6 and CO8 (2.11 and 1.71), which was
followed by T
2
, T
3
, T
4
and T
5
. The lowest Carotenoid
content was recorded in T
9
, T
10
and T
11
treatments
(Table 2). The carotenoid pigments in the leaf tissues
of extreme salinity stress were degraded by 60% and
72% respectively in sugarcane. Reduction in water use
efficiency in this crop under salinity level had a direct
impact on photosynthetic pigment degradation,
leading to reduce water oxidation in photosystem II
(Shao et al., 2008). Total chlorophyll content
decreased when plants are subjected to severe drought
(50% of field capacity), these could be a result of a
reaction centre or a photosystem II modification
(Blum et al., 1989).
2.6 Soluble protein (mg/g)
The soluble protein content of the leaf, being a
measure of RuBP carboxylase activity was considered
as an index for photosynthetic efficiency. There were
reports that RuBP-case enzyme forms nearly 80 per
cent of the soluble proteins in leaves of many plants
(Joseph et al., 1981). The result on soluble protein
content was significantly differed in all the treatments.
Among the treatments, T1 showed highest Soluble
protein content in green gram both CO6 and CO8
(4.19 and 9.16), which was followed by T
3
, T
4
and T
5
.
The lowest Soluble protein content was recorded in T
7
,
T
8
and T
11
treatments (Table 2). Martignone et al. (1987)
observed that in soybean soluble protein content was the
first nitrogenous compound affected under stress
conditions, which at severity got denatured and lost the
activity. It was further explained that soluble protein,
world’s most abundant protein containing the enzyme
RUBISCO, is involved in CO
2
assimilation; therefore, the
reduction in soluble protein might have a direct adverse
effect on photosynthesis.
Table 2 Effect of salt stress (NaCl) on carotenoids and soluble protein content of green gram (CO6 and CO8)
Treatments
Carotenoids (mg/g)
Soluble protein (mg/g)
CO6
CO8
CO6
CO8
T
1
2.11
1.71
4.19
9.16
T
2
2.06
1.66
3.87
8.86
T
3
1.84
1.44
4.47
8.56
T
4
1.82
1.42
4.27
7.34
T
5
1.79
1.39
4.01
7.14
T
6
1.76
1.36
5.49
6.87
T
7
1.74
1.34
2.99
6.68
T
8
1.68
1.28
2.55
6.47
T
9
1.61
1.21
5.91
6.36
T
10
1.56
1.16
3.71
5.64
T
11
1.66
1.26
2.69
6.17
Mean
1.8
1.4
4.0
7.2
SE.d
0.160
0.116
0.418
0.160
CD (0.05)
0.333
0.242
0.867
0.333
3 Materials and Methods
The experiment was carried out at Vanavarayar
Institute of Agriculture (TNAU affiliated), Pollachi,
Tamil Nadu, India during 2013~2014. The experiment
consists of ten treatments with three replications were
laid out in completely randomized block design with
two cultivars of CO5 and CO6. Seeds of green gram
varieties obtained from Department of Pulses, Tamil
Nadu Agricultural University, Coimbatore, were used
for the study and the details of the varietal characters
were listed in Table 3. Green gram varieties (Table 3)
were screened for tolerance to various levels of
sodicity stress, based on germination per cent,
seedling growth and vigour index, seeds were allowed
to germinate in Petri dishes. The germination medium
was prepared following the procedure mentioned
below. Petri dishes were sterilized using 0.01% HgCl
2
and 70% ethanol and finally washed with distilled