基本HTML版本
Genomics and Applied Biology 2014, Vol. 5, No. 4, 1-5
http://gab.biopublisher.ca
4
water. Before placing the germination sheet, Petri
dishes were cleaned thoroughly with a cotton swab.
The surface sterilized (70% ethanol) 15 seeds from
each variety were placed in each Petri dish. For
imposing sodicity (11 levels as considered as
Treatments) stresses, sodium chloride (NaCl) solution
at the concentration of T
1
: control (without NaCl),
T
2
:10, T
3
:20, T
4
:30, T
5
:40, T
6
:50, T
7
:60, T
8
:70, T
9
:80,
T
10
:90 and T
11
:100 ppm were prepared. The seeds
were allowed to germinate, by sprinkling the salt
solution of 10 mL each in alternate days. Distilled
water was used for maintaining the control. The pH
and EC details of the salt solution used for experiment
were given in Table 4.
Table 3 Varietal Details
S.No
Crop
Variety
Duration (days)
Yield (kg/ha)
Rainfed
Irrigated
1.
Green gram
CO6
62~67 days
900
1050
2.
CO8
70 days
900
1050
Table 4 pH and EC of the salt solution used for experiment
S.No
(NaCl) Salt solution
pH
EC (dS/m)
1.
0 ppm
7.1
0.42
2.
10 ppm
7.3
2.0
3.
20 ppm
7.5
4.1
4.
30 ppm
7.6
5.3
5.
40 ppm
7.7
6.9
6.
50 ppm
7.8
7.0
7.
60 ppm
8.2
7.6
8.
70 ppm
8.4
8.1
9.
80 ppm
8.7
8.5
10.
90 ppm
9.2
9.00
11.
100 ppm
9.5
10.00
3.1 Observation recorded
The germination percentage, root length, shoot length
and Vigour index were measured at 15
th
DAS and
chlorophyll ‘a’ ‘b’ and total chlorophyll and
carotenoid content was estimated based on the
procedure given by Yoshida et al. (1979) and
expressed as mg/g fresh weight.
3.2 Photosynthetic pigments
The chlorophyll content was estimated following the
method suggested by Yoshida et al. (1971) and
expressed as mg/g fresh weight.
3.3 Procedure
Take 250 mg of leaf sample is macerated with 10 mL
of 80% acetone using a pestle and mortar and the
extract is centrifuged at 3000 rpm for 10 minutes. The
supernatant solution is transferred into a 25 mL
volumetric flask and made up to 25 mL using 80%
acetone. Then the color intensity of the green pigment
is read at 480 nm, 510 nm, 645 nm, 663 nm and 652
nm for chlorophyll a, chlorophyll b, total chlorophyll
and carotenoid content
respectively using
Spectrophotometer.
3.4 Soluble protein content
Soluble protein content of leaf was estimated as per
the method of Lowry et al. (1951) and expressed as
mg/g fresh weight.
References
Abdual-baki A.A., and J.D. Anderson, 1973, Relationship
between decarboxylation of glutamic acid and vigour in
soybean seed, Crop Sci., 13: 222-226
Blum A., Mayer J. and Andgolan G., 1989, Agronomical and
Physiological assessments of genotypic variation for
drought resistance in sorghum, Australian Journal of
Agricultural Research, 16(1): 49-61
http://dx.doi.org/10.1071/AR9890049
Bray E.A., J. Bailey-Serres and E. Weretilnyk, 2000, Responses