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Genomics and Applied Biology 2014, Vol. 5, No. 4, 1-5
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2
sugar conversion occurs during germination is also
affected by salinity. It also delays the synthesis of
nucleic acids and RNAase. As regard to the
chlorophyll content of the salinized plant, it is
apparent that the chlorophyll content was reduced
with increasing salinity. When salinity has affected the
warning signs were sick or dying trees and declining
vegetation. As salinity impacts on any remaining
native vegetation and the wildlife that depends on it
for survival, the loss of biodiversity escalates. Salinity
also reduces the productivity of crops and the
sustainability of agriculture. Based on the above
constraints, we are taken the objective of Screening of
Green gram varieties for NaCl stress tolerance through
physiological analysis.
2 Result and Discussion
2.1 Photosynthetic pigments
Photosynthetic pigments are composed of
chlorophylls a, b and total, and the main functions are
reception and storage of light energy by inductive
resonance through antenna complexes, and consequent
electron transport carried out by the photosystem II
(Taiz and Zeiger, 2002). Salt stress has been stated to
reason an inhibition of growth and development,
lessening in photosynthesis, respiration and protein
synthesis in sensitive species (Boyer, 1982).
2.2 Chlorophyll a (mg/g)
The result on Chlorophyll ‘a’ was significantly
differed in all the treatments. Among the treatments,
T
1
showed highest Chlorophyll ‘a’ content in green
gram both CO6 and CO8 (3.35 and 1.86), which was
followed by T
2
, T
3
, T
4
, T
5
and T
6
. The lowest
Chlorophyll ‘a’ content was recorded in T
10
and T
11
treatments (Table 1).
2.3 Chlorophyll b (mg/g)
The result on Chlorophyll ‘b’ was significantly
differed in all the treatments. Among the treatments,
T
1
and T
2
showed highest Chlorophyll ‘b’ content in
green gram both CO6 and CO8 (0.78 and 0.32), which
was followed by T
3
, T
4
, T
5
and T
6
. The lowest
Chlorophyll ‘b’ content was recorded in T10 and T11
treatments (Table 1).
Table 1 Effect of salt stress (NaCl) on photosynthetic pigments of green gram (CO6 and CO8)
Treatments
Chlorophyll ‘a’ (mg/g)
Chlorophyll ‘b’ (mg/g)
Total Chlorophyll (mg/g)
CO6
CO8
CO6
CO8
CO6
CO8
T
1
3.35
1.89
0.78
0.32
4.41
2.95
T
2
3.30
1.84
0.78
0.32
4.36
2.90
T
3
3.08
1.62
0.77
0.31
4.13
2.67
T
4
3.06
1.60
0.76
0.30
4.10
2.64
T
5
3.03
1.57
0.74
0.28
4.05
2.59
T
6
3.00
1.54
0.73
0.27
4.01
2.55
T
7
2.98
1.52
0.67
0.21
3.93
2.47
T
8
2.92
1.46
0.66
0.20
3.86
2.40
T
9
2.85
1.39
0.66
0.20
3.79
2.33
T
10
2.80
1.34
0.62
0.16
3.70
2.24
T
11
2.90
1.44
0.65
0.19
3.83
2.37
Mean
3.0
1.6
0.7
0.2
4.0
2.6
SE.d
0.160
0.160
0.034
0.018
0.160
0.160
CD (0.05)
0.333
0.333
0.070
0.039
0.333
0.333
2.4 Total Chlorophyll (mg/g)
The result on total chlorophyll content was
significantly differed in all the treatments. Among the
treatments, T
1
showed highest total chlorophyll
content in green gram both CO6 and CO8 (4.41 and
2.95), which was followed by T
2
, T
3
, T
4
and T
5
. The
lowest total chlorophyll content was recorded in T
9
,
T
11
and T
10
treatments (Table 1).
There was also significantly maintained in the total
chlorophyll content of the seedlings in the T
1
~T
5
treatments as 12.6%, with lesser reduction over the
other treatments. The highest reduction of 25.3
percent was noticed in T
6
-T
12
treated seedlings.
According to Lapina and Popov (1970), saline
conditions lead to disruption of the fine structure of
chlorophyll and instability of the pigment protein