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Legume Genomics and Genetics 2014, Vol. 5, No. 5, 1-5
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2
nucleic acids and RNAase. As regard to the
chlorophyll content of the salinized plant, it is
apparent that the chlorophyll content was reduced
with increasing salinity. When salinity has affected the
warning signs were sick or dying trees and declining
vegetation. As salinity impacts on any remaining
native vegetation and the wildlife that depends on it
for survival, the loss of biodiversity escalates. Salinity
also reduces the productivity of crops and the
sustainability of agriculture. Based on the above
constraints, we are taken the objective of Screening of
Green gram varieties for NaCl stress tolerance through
physiological analysis.
2 Materials and Methods
The experiment was carried out at Vanavarayar
Institute of Agriculture (TNAU affiliated), Pollachi,
Tamil Nadu, India during 2013-2014. The experiment
consists of ten treatments with three replications were
laid out in completely randomized block design with
two cultivars of CO5 and CO6. Seeds of green gram
varieties obtained from Department of Pulses, Tamil
Nadu Agricultural University, Coimbatore, were used
for the study and the details of the varietal characters
were listed in Table 1. Green gram varieties (Table1)
were screened for tolerance to various levels of
sodicity stress, based on germination per cent,
seedling growth and vigour index, seeds were allowed
to germinate in Petri dishes. The germination medium
was prepared following the procedure mentioned
below. Petri dishes were sterilized using 0.01% HgCl
2
and 70% ethanol and finally washed with distilled
water. Before placing the germination sheet, Petri
dishes were cleaned thoroughly with a cotton swab.
The surface sterilized (70% ethanol) 15 seeds from
each variety were placed in each Petri dish. For
imposing sodicity (11 levels as considered as
Treatments) stresses, sodium chloride (NaCl) solution
at the concentration of T
1
: control (without NaCl),
T
2
:10, T
3
:20, T
4
:30, T
5
:40, T
6
:50, T
7
:60, T
8
:70, T
9
:80,
T
10
:90 and T
11
:100 ppm were prepared. The seeds
were allowed to germinate, by sprinkling the salt
solution of 10 mL each in alternate days. Distilled
water was used for maintaining the control. The pH
and EC details of the salt solution used for experiment
were given in Table 2.
Table 1 Varietal Details
S.No
Crop
Variety
Duration (days)
Yield (kg/ha)
Rainfed
Irrigated
1.
Green gram
CO6
62-67 days
900
1050
2.
CO8
70 days
900
1050
Table 2 pH and EC of the salt solution used for experiment
S.No
(NaCl) Salt solution
pH
EC (dS/m)
1.
0 ppm
7.1
0.42
2.
10 ppm
7.3
2.0
3.
20 ppm
7.5
4.1
4.
30 ppm
7.6
5.3
5.
40 ppm
7.7
6.9
6.
50 ppm
7.8
7.0
7.
60 ppm
8.2
7.6
8.
70 ppm
8.4
8.1
9.
80 ppm
8.7
8.5
10.
90 ppm
9.2
9.00
11.
100 ppm
9.5
10.00
2.1 Observation recorded
Soluble protein content: Soluble protein content of
leaf was estimated as per the method of Lowry et al.
(1951) and expressed as mg/g fresh weight.
2.2 Nitrate Reductase enzyme activity
Proline content of the leaf sample was estimated by
the method of Nicholas et al. (1976) and expressed as
µg of NO
2
g
-1
·hr
-1
of fresh weight.
2.3 Proline (mg/g)
Proline content of the leaf sample was estimated by
the method of Bates et al. (1973) and expressed as
µg/g of fresh weight.
2.4 Catalase (mg/g)
Catalase activity of the leaf sample was assayed as per
the procedure adopted by Gopalachari (1963) and
expressed as µg H
2
O
2
g
-1
·min
-1
.