Basic HTML Version
Legume Genomics and Genetics 2014, Vol. 5, No. 5, 1-5
http://lgg.biopublisher.ca
3
3 Result and Discussion
3.1 Soluble protein (mg/g)
The soluble protein content of the leaf, being a
measure of RuBP carboxylase activity was considered
as an index for photosynthetic efficiency. There were
reports that RuBP-case enzyme forms nearly 80 per
cent of the soluble proteins in leaves of many plants
(Joseph et al., 1981). The result on soluble protein
content was significantly differed in all the treatments.
Among the treatments, T1 showed highest Soluble
protein content in green gram both CO6 and CO8
(4.19 and 9.16), which was followed by T
3
, T
4
and T
5
.
The lowest soluble protein content was recorded in T
7
,
T
8
and T
11
treatments (Table 3). Martignone et al.
(1987) observed that in soybean soluble protein
content was the first nitrogenous compound affected
under stress conditions, which at severity got
denatured and lost the activity. It was further
explained that soluble protein, world’s most abundant
protein containing the enzyme RUBISCO, is involved
in CO
2
assimilation; therefore, the reduction in soluble
protein might have a direct adverse effect on
photosynthesis.
3.2 Nitrate Reductase enzyme activity
The result on nitrate reductase activity was
significantly differed in all the treatments. Among the
treatments, T
2
showed highest nitrate reductase
activity in green gram both CO6 and CO8 (83.60 and
85.15), which was followed by T
3
, and T
4
. The lowest
nitrate reductase activity was recorded in T
10
and T
11
treatments (Table 3).
Table 3 Effect of salt stress (NaCl) on soluble protein and nitrate reductase enzyme activity of green gram (CO6 and CO8)
Treatments
Soluble protein (mg/g)
NR-ase enzyme activity (µg NO
2
g
-1
hr
-1
)
CO 6
CO8
CO 6
CO8
T
1
4.19
9.16
59.92
61.91
T
2
3.87
8.86
83.60
85.15
T
3
4.47
8.56
81.04
83.03
T
4
4.27
7.34
80.95
82.94
T
5
4.01
7.14
79.92
81.91
T
6
5.49
6.87
79.25
81.22
T
7
2.99
6.68
77.44
79.43
T
8
2.55
6.47
76.13
78.12
T
9
5.91
6.36
75.25
78.05
T
10
3.71
5.64
64.36
77.24
T
11
2.69
6.17
62.46
66.35
Mean
4.0
7.2
75.8
77.8
SE.d
0.418
0.160
0.160
0.115
CD (0.05)
0.867
0.333
0.333
0.240
3.3 Proline (mg/g)
The result on proline content was significantly
differed in all the treatments. Among the
treatments, T
2
showed highest proline content in
green gram both CO6 and CO8 (382.71 and
384.70), which was followed by T
3
, T
4
, T
5
and T
6
.
The lowest proline content was recorded in T
9
, T
10
and T
11
treatments (Table 4). Rosa - Ibarra and
Maiti (1995) under salinity stress reported that
increase in proline content is probably due to the
capacity of some plants to accumulate organic
(sucrose, fructose and glucose) and inorganic (Na,
K and Cl) metabolites in the cytoplasm to reduce
the water potential and change the osmotic
gradient, assuring the water flow to the plant and
thereby increase tolerance.
3.4 Catalase (mg/g)
The result on catalase activity was significantly
differed in all the treatments. Among the treatments,
T
2
showed highest catalase activity in green gram both
CO6 and CO8 (77.10 and 72.02), which was followed
by T
3
, T
4
and T
5
. The lowest catalase activity was
recorded in T
8
, T
9
, T
10
and T
11
treatments (Table 4). In
this present study, we noticed that, the increase in
enzyme activity with external salinity may be due to
increased synthesis of enzyme. Transgenic plants over
expressing ROS scavenging enzymes, such as super
oxide dismutase (Alscher et al., 2002), ascorbate
peroxidase (Wang et al., 1999) and glutathione