基本HTML版本
昆虫分子生物学研究
(
网络版
), 2013
年
,
第
2
卷
,
第
1-4
页
Kunchong Fenzi Shengwuxue Yanjiu (Online), 2013, Vol.2, 1-4
http://imbr.biopublisher.cn
3
图
2
小菜蛾（
n=31
）基因组连锁图（摘自：
Baxter et al., 2011B
）
注：本图通过
2878
个分离的等位基因构建基因连锁图。每个连锁群包含
10~158
个
RAD
等位基因，图谱总长为
1292 cM
。每个
RAD
标记都用三个
数字
(i_ii_iii)
来标识，相对应为，
i
表示
RAD
标记
(1-285)
，
ii
表示染色体数
(1-31)
，
iii
表示标记中
RAD
等位基因数。连锁群
1-28
与
B. Mori
(n=28)
染
色体编号系统同源，
LG29, LG30
和
LG31
分别代表在
B. Mori
中与染色体
11
，
23
和
24
的融合。图中虚线表示根据因太小而未分组的
3
，
4
或
5
基因
型差异推测出的连锁群。由于有
20
后代个体用于构建此图，每个交叉的大概距离为
5 cM
。在染色体
14
，
16
和
22
上，标记形成了两个明显的组，
因高重组率或染色体分配错误而分隔开。总的来说，总共
285
个
RAD
标记中的
11
个标记并不能确保被分配到预期的染色体。连锁群
28
在单个位点
仅包含
4
个
RAD
标记。从其余的未分配到连锁群的父本标记中，用
JoinMap 3.0
识别出了
6
个额外标记。
Figure 2 Linkage map of the
Plutella xylostella
(n = 31) genome (Adopted from Baxter et al., 2011B)
Note: This was inferred from 2,878 RAD alleles collapsed into 285 discrete RAD markers. Each linkage group contains between 10 and 158 RAD alleles
(labelled RADs) and the total map length is 1,292 cM. Each RAD marker is labelled with three numbers (i_ii_iii) corresponding to (i) the RAD marker (1-285),
(ii) the chromosome number (1-31) and (iii) the number of RAD alleles at that marker. Linkage groups 1-28 are homologous to the
B. mori
(n = 28)
chromosome numbering system, and LG29, LG30 and LG31 represent fusions to chromosomes 11, 23 and 24 respectively in B. mori. Dashed lines represent
manual linkages inferred from 3, 4 or 5 genotype differences that were otherwise left ungrouped due to small sample size. As 20 progeny were used to construct
the map, distances were approximated as 5 cM (1/20) per 1 crossing-over (c/o) event. On chromosomes 14, 16 and 22, markers formed two distinct groups and
may be separated by regions of high recombination rates or chromosomal assignment error. In total, 11 of the 285 RAD markers could not be confidently
assigned to their predicted chromosome. Linkage group 28 contained only four RAD markers at a single locus. Six additional markers were identified for this
chromosome using JoinMap 3.0, from the remaining paternal markers not assigned to linkage groups