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Molecular Soil Biology (online), 2011, Vol. 3 No.2, 5
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8
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Figure 1 Scanning electron microscope (×10 000) and
colony photos of strain SA
-
3
used in the analysis of resilience analysis showed
(Figure 2): In the tough environment of Na
2
CO
3
,
strain SA
-
3 growth occurred in media containing
0~250 mmol/L Na
2
CO
3
and optimal growth were
observed in media containing 30~100 mmol/L
Na
2
CO
3
. On the condition of Na
2
CO
3
not lower
than 30 m we can't find
E.coli
JM109 grows; In
the tough environment of NaCl, strain SA
-
3
growth occurred in media containing 0~2.0 mol/L
NaCl and optimal growth was observed in media
containing 0~0.5 mol/L.
E.coli
JM109 was grew
in the media containing 0.0~0.5 mol/L NaCl,
which is not grown in the media containing up to
0.5~2.0 mol/L NaCl; In the tough environment of
pH value, strain SA
-
3 grew at pH 7.0–11.0, that
showed optimal growth was pH 8.0~9.0,
E.coli
JM109 grew at pH 7~8, severely inhibited the
growth of
E.coli
JM109 grew at pH 9~12. In short,
in the tough environment of Na
2
CO
3
, pH value,
NaCl, the strain SA
-
3 was better than
E.coli
JM109 on the salt and alkali resistances.
1.3 Phylogenetic analysis
Phylogenetic alignment analysis of the partial 16S
rRNA gene sequence (GenBank databases under
Accession Number JF937438) showed that the
isolated SA
-
3 in a distinct lineage with
Nesterenkonia
lutea
YIM 70081
T
within the genus
Nesterenkonia
(Figure 3). These partial 16S rRNA gene sequences
analysis suggested that strain SA
-
3 was most closely
related to
Nesterenkonia lutea
YIM 70081
T
(sequence
consistency: 99.2%),
Nesterenkonia sandarakina
YIM
70009
T
(99.2%),
Nesterenkonia jeotgali
JG-241
T
(99.1%),
Nesterenkonia halotolerans
YIM 70084
T
Figure 2 Resilience analysis of strain SA
-
3 and
E.coli
JM109
Note: The bacteria were grown in liquid medium until OD
600
≈0.6, and then diluted 10
-1
~10
-5
. 3.5 microl liters of each dilution were
inoculated to solid media
Ⅱ
and the LB medium supplemented with different concentrations of Na
2
CO
3
(0~250 mmol/L)
､
NaCl
(0~2.0 mmol/L) and varied pH (7~12). The bacteria were grown at 28
℃
for 48 h with the monitor of the growth. In
E.coli
JM109 as
a control, in addition to NaCl resistance experiments outside the medium pH adjustment 7.0, the remaining conditions are the same culture