Page 6 - Molecular Soil Biology

Molecular Soil Biology (online), 2011, Vol. 3 No.2, 5-8
http://msb.sophiapublisher.com
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Figure 3 16S rRNA sequence phylogenetic tree
Note: Phylogenetic trees showing the relationships between strain SA- 3 and related bacteria based on 16S rRNA gene sequences
alignment analysis. Bootstrap values from 1 000 replicates are included, the scale bar corresponds to 0.005-estimated nucleotide
substitution per sequence
(99.0%), Nesterenkonia aethiopica DSM 17733T
(97.5%), the highest similarity, higher than 97%. The
results show that the strain SA- 3 is belonged to genus
Nesterenkonia.
Summary, the strain SA-3 was isolated from Extreme
soda saline-alkali soil. It was Gram-positive, catalase-
positive, and oxidase-negative and formed Orange
colonies, and starch is not hydrolyzed, which is a
member of the genus Nesterenkonia. In E.coli JM109
as a control, in the tough environment of Na2CO3, pH
value, NaCl, the strain SA-3 was better than E.coli
JM109 on the salt and alkali resistances. The strain
SA -3 is salt-tolerant alkaliphilic actinomycetes. The
salt-tolerant alkaliphilic actinomycetes is a particular
taxa of actinomycete grow in extreme environment,
which can produce alkaline proteases, amylases,
cellulases and lipases that having unique properties
under extreme conditions. These biocatalysts Widely
used in industrial production. Wherefore the strain
SA -3 have a great potentiality to develop.
3 Materials and Methods
3.1 Micro-organisms and culture conditions
In the couse of the survey of cultured bacteria of
alkaline environments in heilongjiang, northeastern