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Molecular Soil Biology (online), 2011, Vol. 3 No.2, 5
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8
http://msb.sophiapublisher.com
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Research Letter Open Access
Isolation and Characterization of An Alkaliphilic and Halotolerant
Nesterenkonia.sp
from An extreme Soda Saline-alkali Soil in the Northeastern of China
Wei Shi
1
, Takano Tetsuo
2
, Shenkui Liu
1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education,; Alkali Soil Natural Environmental Science
Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, 150040;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Corresponding author email:
shenkuiliu@nefu.edu.cn;
Author
Molecular Soil Biology, 2011, Vol.3 No.2 doi: 10.5376/msb.2011.03.0002
Received: 29, Jun., 2011
Accepted: 01, Aug., 2011
Published: 09, Aug., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Shi et al., 2011, solation and characterization of an alkaliphilic and halotolerant
Nesterenkonia.sp
from an extreme soda saline-alkali soil in the northeastern of
China, Molecular Soil Biology, doi:10.5376/msb.2011.03.0002
Abstract
A strain of alkaliphilic and halotolerant, rod-shaped bacterium, designated strain SA
-
3, was solated from an Extreme
soda saline-alkali soil in Heilongjiang, northeastern China. Strain SA
-
3 was Gram-positive, catalase-positive, and oxidase-negative
and formed Orange colonies, and starch is not hydrolyzed, optimal growth conditions were pH 9.0 and 0.5 mol/L NaCl. Strain SA
-
3
has strong resistance to Na
2
CO
3
, hight pH value, and NaCl. 16S rRNA a gene sequence alignment analysis show that the strain
formed a separated line within a clade containing the genus
Nesterenkonia
in the phylum Actinobacteria and was related to the
species
Nesterenkonia
lutea YIM 70081
T
(sequence similarity 99.2%),
Nesterenkonia sandarakina
YIM 70009
T
(99.2%),
Nesterenkonia jeotgali
JG
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241
T
(99.1%),
Nesterenkonia halotolerans
YIM 70084
T
(99.0%),
Nesterenkonia aethiopica
DSM 17733
T
(97.5%). This similarity, suggest that this salt-tolerant alkaliphilic bacterium belonged to genus
Nesterenkonia
. This strain is a great
potential microbial resources for development.
Keywords
Nesterenkonia
; 16S rDNA sequence
Background
Nesterenkonia
as a genus was put forward by
Stackebrandt (Stackebrandt et al., 1995). Which be
classified as which be classified as one genus of
common bacterial form and hight G+C content
Actinobacteria, Currently, the genus contai eleven
species:
Nesterenkonia halobia