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Molecular Plant Breeding 2012, Vol.3, No.2, 11
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Table 5 Estimates of different diversity parameters involving
genotypes of
C. capsularis
(152) and
C. olitorius
(140) using
polymorphic SSR loci
Collection of genotypes
N
N
a
N
e
I
H
e
C. capsularis
Indigenous
101 2.619 1.567 0.492 0.289
Exotic
51 2.08 1.584 0.462 0.282
Total
152
Average
2.35 1.575 0.477 0.286
C. olitorius
Indigenous
106 2.393 1.445 0.415 0.245
Exotic
34 2.107 1.530 0.454 0.278
Total
140
Average
2.25 1.487 0.435 0.262
Note:
N
, sample size;
N
a
, mean no. of observed alleles per
locus;
N
e
, mean no. of effective alleles per locus;
I
, mean
values of Shanon’s information index per locus;
H
e
, mean
expected heterozygosity per locus
Figure 5; Table 9). Of the 152 genotypes of
C.
capsularis
, 134 genotypes were placed in one of the
two sub-populations (CI and CII), while 18 genotypes
were categorized as admixed forms (the criteria
followed here was discussed in Material and methods).
Sub-population 1 (CI) of
C. capsularis
consisted of 49
genotypes among which maximum number of 17
genotypes were from Thailand. In sub-population 2
(CII), all the 85 genotypes except four were indigenous.
The majority (14; 77.78%) of the admixed genotypes
were also indigenous.
In
C. olitorius
, 116 genotypes out of 140 were
grouped into two sub-populations namely OI and OII
containing 70 and 46 genotypes, respectively. The
remaining 24 genotypes were classified as admixed
genotypes. The majority of genotypes (44 out of 70) in
OI sub-population and all the 46 genotypes in OII
sub-population were of Indian origin; the remaining
26 genotypes in sub-population OI belonged to six
other countries (Table 9). The pair wise Nei’s unbiased
genetic distance (GD) and population differentiation
(
Fst
) values between the two sub-population (CI and
CII) of
C. capsularis
were 0.086 and 0.070, respectively
Table 6 Alleles specifically present in indigenous and exotic collection of genotypes of
C. capsularis
and
C. olitorius
Collection
of
genotypes
C. capsularis
C. olitorius
Total no. of specific
alleles (%)
No. of loci
(%)
No. of loci shared
between collections
Total no. of specific
alleles present (%)
No. of loci
(%)
No. of loci shared
between collections
Indigenous 74 (23.94%)
64 (56.64%) 2
63 (17.60%)
55 (39.30%) 7
Exotic
13 (4.21%)
9 (7.97%)
23 (6.42%)
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