Basic HTML Version
Molecular Plant Breeding 2012, Vol.3, No.2, 11
-
25
http://mpb.sophiapublisher.com
16
Table 3 Details of genotype specific alleles identified among
the genotypes of
C. capsularis
and
C. olitorius
Genotypes and country of origin
SSR markers and
alleles (bp)
C. capsularis
CIJ 071 (Nepal)
MJM522 (343)
CEX 051 (Srilanka)
MJM78b (303)
Bidhan Pat
-
2 (India)
MJM429 (300)
MJM540 (202)
C. olitorius
OIJ 277 (Nepal)
MJM944 (258)
OIJ 109 (Tanzania)
MJM1149 (235)
MJM1095 (172)
OIJ 151 (Kenya)
MJM1099 (259)
Bidhan Rupali (India) MJM1130 (196)
OIM 012 (India)
MJM257 (204)
MJM385 (327)
JRO 066 (India)
MJM294 (279)
OIM 025 (India)
HK-63 (130)
and
H
e
(except
N
e
) were higher in the indigenous
genotypes than in the exotic genotypes belonging to
C. capsularis
. In
C. olitorius
the exotic genotypes
showed slightly higher values of
N
e
,
I
and
H
e
than the
indigenous genotypes (Table 5). The above study
revealed that the exotic genotypes were more diverse
than the indigenous genotypes of
C. olitorius
and the
reverse situation was observed in the case of
C.
capsularis
.
Further analyses were conducted on specific alleles
which were present in either indigenous or exotic
collection; these are described as private alleles (Table
6; Table 7). Moreover, in
C. capsularis
24.13% of
private alleles and in
C. olitorius
25.58% of private
alleles (Table 7) showed allele frequency greater than
6%. The number of private alleles for exotic collection
was higher in
C. olitorius
than in
C. capsularis
. Also,
only few SSR loci were shared between indigenous
and exotic collections which are higher in
C. olitorius
(Table 6).
The pair-wise values for unbiased genetic distance
(GD), genetic identity (GI), population differentiation
(
Fst
) and gene flow (
Nm
) between the indigenous and
exotic collections of both the species are presented in
Table 8. The data clearly showed that there is low
genetic distance (GD) and population differentiation
(
Fst
) between the indigenous and exotic genotypes of
both the species. However, population differentiation
between the indigenous and exotic genotypes was
slightly higher in
C. capsularis
, than in
C. olitorius
.
Correspondingly, higher level of gene flow existed
between the indigenous and exotic collection in
C.
olitorius
(Nm = 5.206) than in
C. capsularis
(Nm =
3.672) which confirmed the AMOVA results.
1.6 Population Structure
The Bayesian model-based approach to determine
population structure in the two species of jute with
STRUCTURE software (Pritchard et al., 2000) revealed
two sub-populations (i.e.
K
= 2) in each of the two
species (
C. capsularis
and
C. olitorius
, Figure 4;
Table 4 AMOVA for all jute genotypes and separately for the genotypes in two species based different set of SSR primers
Source of variation
df
SS
MS
Estimated Variance % of total variance
Probability
Two jute species
Among species
1 29 501.102
29 501.102 201.586
63
Within species
290 34 584.665 119.257
119.257
37
P<0.001
Total
291 64 085.767
320.843
100
C.capsularis
Indigenous and Exotic collection
Between collections
1
842.996 842.996
11.175
12
Within collection
150 12 837.715
85.585
85.585
88
P<0.001
Total
151 13 680.711
96.76
100
C. olitorius
Indigenous and exotic collection
Between collections
1
698.123 698.123
11.557
10
Within collection
138 14 228.906 103.108
103.108
90
P<0.001
Total
139 14 927.029
114.665
100