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Wang et al., 2011, Genetic diversity of the selected 64 potato germplasms revealed by AFLPmarker, Molecular Plant Breeding Vol.2 No.4 (doi: 10.5376/mpb.2011.02.0004)
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scientist Zabeau and Vos in 1993. Recent years, AFLP
has been thoroughly applied in potato breeding
(Iovene and Barone, 2004; Tae-Ho, 2005; Riccardo et
al., 2007; Björn B. D’hoop et al., 2008; Zhang et al.,
2009; Li et al., 2007; Di et al., 2006). Meanwhile, it
was gradually applied in genetic relationship and
genetic diversity of many plants, such as
Erianthus
(Liu et al., 2009),
Tea
(Ji et al., 2009),
Motherwort
(Yu et al., 2009),
longan
(Peng et al., 2008),
Naked
oat
(Xu et al., 2009),
Jujube
(Qiao et al., 2009),
Cane
(Lao et al., 2008),
Evodia
(Huang et al., 2008). The
conservation of Chinese potato is little and also the
genetic basis of it is narrow. Introduction and
collection of potato germplasms are extremely vital,
and further define the relationship between these
resources and Chinese potato varieties is the premise
of making full use of the introduction of resources.
Give a broad overview of potato genetic diversity
research in China, the results showed that Chinese
potato genetic basis was narrow, and examined potato
materials were limited to some domestic cultivars. In
this study, the genetic diversity of International Potato
Center (CIP) resources and some potato varieties in
Qinghai Province were analyzed by AFLP markers.
Selecting parents with genetic differences in
hybridizing will be able to broaden the genetic basis,
increase the heterozygosity of breeding populations,
maintain a rich population polymorphic and provide
parent materials with distant genetic relationship for
further study of potato breeding. The aims of this
study are: determine the genetic distance between the
examined potato materials and to know genetic
relationships of CIP resources and Chinese potato
cultivars.
1 Results
1.1 Polymorphic Analysis
64 potato materials were amplified with 12 pairs of
selected AFLP primer combinations. Different primer
combinations had differences in the number of
fragments, length of fragment and percentage of
polymorphism. 12 pairs of primers amplified a total of
505 bands with a mean of 42 bands, of which 388
were polymorphic bands. Average polymorphism rate
(PR) was up to 76.28%, indicating that AFLP markers
can be used to analysis the genetic diversity of potato
efficiently. The amplified bands were between 33 for
E42M48 and 56 for E42M50 with an average of 52 per
primer pair (Table 1). The Poly-morphisms Rates (PR)
varied from 64.29% for E42M50 to 94.44% for E36M59
(Table 1) with an average of 76.28%. The selective
amplification results of 64 potato varieties in table 1.
Using POPGENE 32, Shannon’s Information Index (I)
and Nei’s Gene Diversity Index (H) of tested potato
and 0.4988 with an average of 0.3745, Nei’s Gene
germplasms were obtained (Table 1). Shannon’s
Information Index of materials was between 0.2456
Table 1 The results of amplification of 64 potato materials
Primer Combination
NPB*
Total Bands
PR* (%)
H*
I*
E42M50
36.00
56.00
64.29
0.299 7 (0.170 1)
0.451 7 (0.227 0)
E36M54
28.00
35.00
80.00
0.193 6 (0.185 2)
0.303 7 (0.256 2)
E42M48
22.00
33.00
66.67
0.151 0 (0.161 5)
0.245 6 (0.234 1)
E38M48
31.00
41.00
75.61
0.207 9 (0.189 3)
0.321 7 (0.262 3)
E35M62
24.00
34.00
70.59
0.319 4 (0.176 0)
0.478 0 (0.221 9)
E42M59
31.00
42.00
73.81
0.272 8 (0.179 3)
0.417 4 (0.234 6)
E36M48
29.00
36.00
80.56
0.234 2 (0.173 6)
0.368 4 (0.231 1)
E39M54
24.00
40.00
60.00
0.160 1 (0.186 0)
0.249 1 (0.264 4)
E39M59
47.00
51.00
92.16
0.246 8 (0.185 3)
0.495 0 (0.212 3)
E36M61
35.00
40.00
87.50
0.225 1 (0.158 2)
0.357 9 (0.219 5)
E36M59
51.00
54.00
94.44
0.325 6 (0.179 5)
0.498 8 (0.245 5)
E39M49
30.00
43.00
69.77
0.194 0 (0.184 5)
0.307 1 (0.260 4)
Mean
32.33
42.08
76.28
0.235 9 (0.177 6)
0.374 5 (0.239 7)
Total
388.00
505.00
Note: *NPB is the number of polymorphic bands, PR is the polymorphism rate of primers, H is Nei’s gene diversity index, I is
Shannon’s information index