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Molecular Plant Breeding 2011, Vol.2, No.4, 22
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Research Report Open Access
Genetic Diversity of the Selected 64 Potato Germplasms Revealed by AFLP
Markers
Fang Wang
1, 2
, Fangdi Li
1, 2, 3
, Jian Wang
1, 2
, Yun Zhou
1, 2
, Haihong Sun
1, 2
1.Institute of Biotechnology of Qinghai Academy of Agriculture and Forestry, Xining, 810016, P.R. China
2.The Qinghai-Tibet Plateau Biotechnology Key Lab of Ministry of Education, Xining, 810016, P.R. China
3.Qinghai University, Xining, 810016, P.R. China
Corresponding author email: wangjian2197@sohu.com;
Author
Molecular Plant Breeding, 2011, Vol.2 No.4 doi: 10.5376/mpb.2011.02.0004
Received: 28, Dec., 2010
Accepted: 06, Jan., 2011
Published: 25, Feb., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wang et al., 2011, Genetic diversity of the selected 64 potato germplasms revealed by AFLP marker, Molecular Plant Breeding Vol.2 No.4 (doi:
10.5376/mpb.2011.02.0004)
Abstract
In order to utilize the elite germplasms from the International Potato Center (CIP) for potato breeding program in China,
we analyzed the genetic relationship of selected 64 potato cultivars based on the AFLP approach in this study. Twelve polymorphic
primer combinations were screened out to be used for analyzing the genetic diversities among the tested materials. The number of
bands generated by each selected AFLP primer pair reached about 42 in the ranged of from 33 to 56. Total of 505 reproducible bands
were amplified by 12 primer, of which polymorphic bands were 388. The genetic distance were calculated among the tested 64 potato
cultivars in the range from 0.041 237 1 to 0.391 732 6. The cluster analysis showed that domestic cultivars were assigned to be a
group at the genetic coefficient of 0.82 and all tested potato cultivars were clustered together at the genetic similarity coefficient (GS)
of 0.68. Shannon’s Information Index of examined materials was between 0.245 6 and 0.498 8 with an average of 0.374 5, as well as
Nei’s Gene Diversity Index of examined materials varied from 0.151 0 to 0.325 6 with a mean of 0.235 9. In this research, we might
have a conclusion that that genetic basis of domestic potato cultivars is quite narrow compared to the accessions of potato resources
from CIP. Therefore, the CIP germplasms can be used for broadening the genetic basis of local potato cultivar in China.
Keywords
Potato; Potato Germplasms; Genetic Diversity; AFLP
Background
Currently, the cultivation of potato is mainly
depending on cross breeding. The selection of parent
materials is the key of breeding. Determination of
genetic distance and genetic relationship is one of
crucial factors to be considered in matching of parents.
More genetic diversity existed between the parents
would breed new cultivars with resistance to disease
longer-lasting. Previously, parent materials were
identified by analyzing the genetic distance of
morphological characteristics. Influenced by internal
and external environmental factors, quantitative
characters, dominant effect and other influential
factors easily, the traditional method had inevitable
deviations. The molecular markers due to be uneasy to
be affected by environmental factors, and larger
quantities, can be quickly and accurately distinguish
the genetic relationship of parent materials.
With developing of molecular marker technology
rapidly, molecular markers have been used as a
routine tool to penetrate into all areas of biological
research and produce an enormous impact on the
practice of crop breeding. Molecular markers were
used to analysis potato genetic diversity earlier in
foreign countries (Kardolus et al., 1998; McGregor,
2002; cGregor et al., 2000; John Bamberg et al., 2008;
Atul Grover et al., 2009). Compared with foreign
countries, molecular markers were used in Chinese
potato breeding lately, but in recent years, the
molecular marker has gradually applied to analysis
genetic diversity of potato in China (He et al., 2007;
Li et al., 2000; Liu et al., 2004; Sun et al., 2006; Li et
al., 2007; Xu et al., 2007; Duan et al., 2009). AFLP
(Amplified fragment length polymorphism) is a new
type of DNA molecular markers which was created
and continuously developed by the Netherlands