Basic HTML Version
Legume Genomics and Genetics (online), 2010, Vol. 1, No.7, 34-40
http://lgg.sophiapublisher.com
Figure 3 Plant height mutant in M
3
generation of azuki bean
Note: A: JN6 CK; B: Height mutant
ned with the treatment of 0.4% colchicine.
1.2.3 Pod color and seed trait mutation
JN 6 has white pods, but 15 black pod mutants appeared
in 0.4% colchicine-induced M
3
(Figure 4), and the mu-
tation rate was 1.5%.
Seed colour of JN6 is russetish, while mutations of black
red and light red seeds were found in M
3
. The average
values of L*, b* and a* for different seed color mutants
were measured with Color Difference Meter (Table 6).
Additionally, the pod color and seed color were closely
related. The mutants with black red seed color were
ordinarily those with black pod (Figure 5), and all black
red seed mutants were obtained by the treatment with
0.4% colchicine.
Figure 4 Pod color mutant of in bean M
3
Note: A: JN6 Control; B: Black pod
Table 6 L*, a* and b* of seed colour mutant
Seed color
L*
a*
b*
Black red seeds
19.75
13.16
4.34
Light red seeds
24.92
17.54
8.23
JN6 Control
22.54
15.72
6.44
Figure 5 Seed color mutant in bean M
3
Note: A: JN6 Control; B; C: Light red seeds; D: Black red seeds; E:
Yellow-white seed
The seed of JN6 is smooth and column with the average
100
-
seed weight of 17.67 g. 3 big-seed mutants were ga-
ined in M
3
population induced by 0.4% colchicine (Figure
6). The average 100
-
seed weight of big-seed mutant was
37.12 g, increased by 47.39% compared with the control.
Figure 6 Seed shape mutant of in bean M
3
Note: A: JN6 Control; B: Big seed
1.2.4 Variation of growth period
The growth period of JN6 is usually from 90 to 95 days.
9 early-maturing mutants were found in M
3
, earlier from
10 to 15 days than the control. 8 late-maturing mutants
were later from 7 to 10 days than the control.
1.3 Analysis on trait stability of mutants in M
4
ge-
neration
Mutant traits were investigated in M
3
and M
4
. Trait
stability of 208 mutant lines in total involving 17 varia-
38