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Legume Genomics and Genetics (online), 2010, Vol. 1, No.7, 34-40
http://lgg.sophiapublisher.com
Table 5 The variation types of M
3
induced by colchicines in Jingnong6 of Azuki bean
0.4% colchicine (12 h)
0.2% colchicine (12 h)
Type of variation
No. of mutants
Frequency of mutants
No. of mutants
Frequency of mutants
Deep-green leaf
9
0.90
2
0.20
Light-green leaf
8
0.80
0
0.00
New leaf yellowing
3
0.30
2
0.20
Yellow spot leaf
4
0.40
1
0.10
Sword leaf
19
1.90
0
0.00
Kidney leaf
10
1.00
17
1.70
Small heart-shape leaf 12
1.20
6
0.60
Leaf trait
Glave kidney leaf
7
0.70
0
0.00
Higher plants
10
1.00
0
0.00
Tight type
5
0.50
1
0.10
Excessive branches
2
0.20
1
0.10
Few branches
7
0.70
0
0.00
Thick stalk
2
0.20
0
0.00
Definite growth
34
3.40
25
2.50
Plant
architecture
Sprawl mutant
2
0.20
1
0.10
Black pod
15
1.50
0
0.00
Plenty pods
8
0.80
2
0.20
Pod trait
Yellow-white seed
1
0.10
0
0.00
Light red seeds
5
0.50
12
1.20
Dark red seeds
10
1.00
0
0.00
Seed trait
Big seeds
3
0.30
0
0.00
Late maturing
8
0.80
1
0.10
Maturing
trait
Early maturing
6
0.60
3
0.30
Total
189
18.90
74
7.40
Figure 2 Leaf color mutants in M
3
generation of azuki bean
Note: A: JN6 Control; B: Deep-green leaf; C: Light-green leaf; D: New leaf yellowing; E: Yellow spot leaf
1.2.2 Plant trait variation
In colchicine-treated M
3
population, sprawl variant
appeared with no significant differences in yield factors
compared with the control. Vertical, sturdy and compact
plant types got in the 0.4% colchicine-treated M
3
.
Plant height of JN6 is between 40 cm and 60 cm in ge-
neral, with the average of 42.51 cm. However, in 0.4%
colchicine-induced M
3
populations, 10 plants with the
height between 70 and 87 cm were produced (Figure 3),
and the average height was 78.05 cm, increased by 83.6%
than the control.
Significant variations occurred in branch number of
plants such as excessive branch and few branch in M
3
.
JN 6 Variety generally had 3 or 4 branches, while, 3
mutants with 8 branches and 7 few-branch variants with
1 branch appeared. All few-branch mutants were obtai-
37