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Genomics and Applied Biology, 2010, Vol.1 No.2
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Figure 4 Neighbor-joining tree based on the deduced amino
acid sequences of
HD
homologs
Note: Sequences were shown by their accession numbers (locus
tags) and strain names; Bootstrap values from neighbor-joining
analyses were listed to the left of each node, with values more
than 50 were shown
Figure 6 Neighbor-joining tree based on the deduced amino
acid sequences of
ENR
homologs
Note: Sequences were shown by their accession numbers (locus
tags) and strain names; Bootstrap values from neighbor-joining
analyses were listed to the left of each node, with values more
than 50 were shown
2 Discussion
Peanut is an important crop internationally for both
food and oil production (Luo et al., 2005). One of the
major factors influencing peanut oil quality is the
Figure 7 Expression analysis of
AhKR
,
AhHD
and
AhENR
genes in four different tissues
composition of fatty acid. So validating the
mechanism of the fatty acid synthesis and metabolism
is the central goal to increase peanut quality. Fatty
acid biosynthesis in higher plants is carried out by
type II FAS. In the present study, we isolated
AhKR
,
AhHD
and
AhENR
orthologues in peanut seedling.
Sequence comparisons revealed that the primary
structure of plant plastid type II FAS enzymes (KR,
HD, ENR) was strictly conserved, especially the
catalytic residues, which suggested that these enzymes
may have similar functions in higher plants as those in
E. coli
. Real-time RT-PCR analysis revealed that the
three genes were expressed dominantly in leaf among
four tissues tested, and expressed at the lowest level in
stem.
AhKR
and
AhENR
genes shared similar
expression behaviors over the developmental stages
compared to that of
AhHD
gene. These results indicated