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Bioscience Methods 2012, Vol.3, No.2, 7
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20
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(0.1 mg/L), BAP (0.5 mg/L and 1.0 mg/L), kinetin
(0.25 mg/L) and proline (250 mg/L) were used. Calli
of 3 different ages, viz., 21 days, 28 days and 35 days
were used for regeneration experiment. Calli of both
genotypes, those were induced on CFM3 and CFM11,
shifted on four regeneration media (RM). Data
were collected in the form of total number of shoots
per explants. Analysis of variance for regeneration
showed that the interaction between days, genotype,
CFM and RM is highly significant (Table 3).
Among genotypes, the genotype S
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2003
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us
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127
produced more number of shoots per explant. Calli of
28 days from CFM11 produced 380 shoots per explant
in genotype S
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2003
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us
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127 on RM2 (Figure 5). This
proved to be the best combination for regeneration;
followed by 35 days old calli of this genotype on the
same medium (RM2) gave 136.67 shoots per explant.
Calli of age 35 days from CFM3 gave 73 shoots per
explants on RM1, in genotype S
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2003
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us
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127 followed
by 28 days old calli of this genotype on the same
regeneration media (RM1) produced 31.67 shoots
per explants (Table 4; Figure 5). Contrary to this, the
Table 3 Analysis of variance table for regeneration: Statistical analysis (ANOVA) for Regeneration in different sugarcane genotypes
on different RM
Source of variation
Degrees of freedom
Sum of squares
Mean squares
F-value
Days
2
49 124.042
24 562.021
39.85
**
Genotype
1
61 297.507
61 297.507
99.45
**
Days×Genotypes
2
36 548.931
18 274.465
29.64**
CFM
1
65 749.507
65 749.507
106.67
**
Days×CFM
2
50 779.347
25 389.674
41.19
**
Genotype×CFM
1
29 842.563
29 842.563
48.41
**
Days×Genotype×CFM
2
41 821.292
20 910.646
33.92
**
Regen. Media (RM)
3
31 122.021
10 374.007
16.83
**
Days×RM
6
17 378.292
2 896.382
4.69
**
Genotype×RM
3
38 237.188
12 745.729
20.67
**
Days×Genotype×RM
6
16 916.958
2 819.493
4.57
**
CFM×RM
3
33 178.188
11 059.396
17.94
**
Days×CFM×RM
6
23 106.208
3 851.035
6.24
**
Genotype×CFM×RM
3
35 131.243
11 710.414
18.99
**
Days×Genotype×CFM×RM
6
19 850.486
3 308.414
5.36
**
Error
96
59 170.667
616.361
Total
143
609 254.438
Note: **: Highly significant (P<0.01)
Figure 5 Regeneration response of sugarcane genotypes on different regeneration media (RM)
Note: 28 days old calli of genotype S
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2003
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us
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127 gave maximum shoot formation on RM2 (0.1 mg/L 2,4
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D and 1 mg/L BAP)