Page 10 - BM 2012 vol.3 No.2

Bioscience Methods 2012, Vol.3, No.2, 7-20
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Table 4 Means for interaction among Days×Genotype×CFM×RM
Genotype CFM RM Day
21 Days 28 Days 35 Days
S-2003- us-127 CFM11 RM1 36.33±3.48 def 116.33±7.80 b 51.00±5.86 de
RM2 40.00±7.51 def 380.00±96.1 a 136.67±5.49 b
RM3 0.00±0.00 f 108.67±6.96 bc 8.00±2.52 ef
RM4 10.00±2.08 ef 118.33±9.56 b 2.67±0.88 ef
CFM3 RM1 3.00±0.58 ef 31.67±9.53 def 73.00±6.81 cd
RM2 18.33±2.73 ef 3.33±0.88 ef 4.00±1.73 ef
RM3 0.00±0.00 f 0.00±0.00 f 8.00±2.52 ef
RM4 0.00±0.00 f 0.00±0.00 f 8.33±2.19 ef
S-2003- us-371 CFM11 RM1 2.00±0.58 ef 11.67±2.33 ef 3.33±2.03 ef
RM2 1.67±0.33 ef 23.33±3.18 ef 10.00±3.51 ef
RM3 1.33±0.33 ef 1.33±0.88 ef 26.67±4.67 def
RM4 3.33±0.88 ef 36.67±7.31 def 46.00±3.79 def
CFM3 RM1 0.00±0.00 f 0.00±0.00 f 0.00±0.00 f
RM2 0.00±0.00 f 0.00±0.00 f 0.00±0.00 f
RM3 0.00±0.00 f 0.00±0.00 f 0.00±0.00 f
RM4 0.00±0.00 f 0.00±0.00 f 0.00±0.00 f
Note: Table 4 means sharing similar letter are statistically non-significant (P>0.05)
genotype S-2003-us -371, gave maximum number of
shoots on RM4. Thirty five (35) days old calli from
CFM11 produced 46 shoots per explant on RM4
followed by 28 days old calli from CFM11 gave 36.67
shoots per explant on the same regeneration medium
(RM4).
Genotype S- 2003-us-371showed no regeneration
response, when calli from CFM3 were shifted to all
regeneration media (RM), just calli proliferation was
observed, and callus mass was increased with no
regeneration response (Figure 6).
Figure 6 Calli of CFM3 was being continuously proliferated
and mass was increased on all four regeneration media in
genotype S-2003- us-371
Albino plants formation was observed when calli of
genotype S-2003-us- 371 from CFM11 were shifted
to RM2 (Figure 7). Whitish pink shoots were formed
Figure 7 Formation of shoots with whitish pink colour in
genotype S- 2003-us- 371, when calli induced on CFM11 were
transferred to RM2
on this regeneration medium.
Different regeneration stages of both genotypes
(S- 2003-us -127 and S-2003-us-371) on regeneration
media as well as root induction medium can be seen in
Figure 8 and Figure 9.
In vitro regenerated plants of both genotypes were
acclimatized in green house. Acclimatization of these
in vitro regenerated under green house condition can
be seen in Figure 10.
1.4 Genetic stability determination
Genetic stability determination is very crucial for in
vitro regenerated plants of selected media combination.
For genetic transformation, it is necessary that the