Page 8 - BM 2012 vol.3 No.2

Bioscience Methods 2012, Vol.3, No.2, 7-20
http://bm.sophiapublisher.com
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Figure 2 Callus formation response of sugarcane genotypes on different callus formation media (CFM)
Note: Genotype S-2003- us-127 gave highest callus mass on CFM3 (3 mg/L 2,4 - D), CFM11 (3 mg/L 2,4-D, 0.1 mg/L Kinetin) and
CFM12 (3 mg/L 2,4- D, 0.1 mg/L Kinetin), the most effective media for callus induction
Table 2 Mean interaction of Genotypes and CFM
Callus formation media CFM Genotype Overall mean of CFM
S- 2003-us- 127 S-2003- us-371
CFM1 2.00±0.00 d 2.00±0.00 d 2.00±0.00 E
CFM2 3.00±0.58 c 2.00±0.00 d 2.50±0.34 D
CFM3 5.00±0.00 a 4.00±0.58 b 4.50±0.34 A
CFM4 5.00±0.00 a 2.00±0.00 d 3.50±0.67 B
CFM5 0.01±0.00 f 1.00±0.00 e 0.51±0.22 F
CFM6 0.01±0.00 f 1.00±0.00 e 0.51±0.22 F
CFM7 0.01±0.00 f 1.00±0.00 e 0.51±0.22 F
CFM8 0.01±0.00 f 1.00±0.00 e 0.51±0.22 F
CFM9 3.00±0.00 c 3.00±0.00 c 3.00±0.00 C
CFM10 4.00±0.00 b 3.00±0.00 c 3.50±0.22 B
CFM11 5.00±0.00 a 4.00±0.00 b 4.50±0.22 A
CFM12 5.00±0.29 a 4.00±0.58 b 4.50±0.37 A
Overall means of genotypes 2.67±0.36 A 2.33±0.21 B
Note: Table 2 means sharing similar letter in a row or in a column are statistically non-significant P>0.05; Small letters represent
comparison among interaction means and capital letters are used for overall mean
Figure 3 Stages of calli of genotype S- 2003-us- 127
produced maximum callus. Despite of an excellent
callus formation response of both genotypes also on
CFM12, this medium was rejected because high level
of 2,4- D might disrupt genetic stability of in
Figure 4 Stages of calli of genotype S- 2003-us- 371
vitro regenerants which is significantly not desired for
genetic transformation approaches. For regeneration
study, four regeneration media (RM) were used in
which different plant growth regulators viz., 2,4-D