Page 11 - BM 2012 vol.3 No.2

Bioscience Methods 2012, Vol.3, No.2, 7-20
http://bm.sophiapublisher.com
14
Figure 8 Different stages of regenaration of both genotypes
Note: A, B, C and D= Regeneration response of genotypes
S-2003- us-127 from shoot emergence from callus to complete
plantlets formation; E, F, G and H= Regeneration response of
genotypes S-2003- us-371 from shoot emergence from callus
to complete plantlets formation
variation could only induce through genetic transfor-
mation and not due to tissue culture regime. For this
purpose, five random primers were used. All Random
Amplified Polymorphic DNA primers showed genetic
stability and same banding pattern was observed in
regenerants and wild type (Figure 11).
Figure 9 Plantlets of both genotypes on root induction medium
(1/2MS)
Note: A and B: Genotypes S -2003-us- 127, C and D: Genotypes
S- 2003-us- 371
Figure 10 Acclimatization of in vitro regenerated plants under
greenhouse conditions
Figure 11 GL decamer K- 07
Note: M: 1 kb ladder; E: Empty; WT: Wild type plant; P1, P2:
In vitro regenerated plants of selected media combination
2 Discussion
An efficient and reproducible regeneration system is
indispensible for genetic transformation to improve
the yield potential and resistance for biotic and abiotic
stresses. Callus is the most suitable target for the