Molecular Plant Breeding 2016, Vol.7, No.29, 1
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single genotype. For convenient understanding of the clustering pattern, a three dimensional scattered diagram
was formed based on PCA score I, II, and III obtaining from the principal component analysis which accounted
for 96.6% toward to total variation (Figure 1). Scattered diagram also showed 6 distinctive clustering patterns. The
clustering pattern comprising of the 30 genotypes from different sources indicated that there was no association
between eco geographical distribution of genotypes and genetic divergence. Similar result was found by Indra et
al
.
(2000), Sreelathakumary and Rajmony (2004), Farhad et al. (2010), Datta and Jana (2011), Datta and Das
(2013) and Yatung et al. (2014).
Figure 1 Three dimensional scattered diagram based on three PCA scores showing the distribution of different genotypes
1.2 Inter and intra-cluster distances
The average intra and inter cluster distances were presented in Table 1. The highest intra cluster divergence
(54.519) for cluster VI was smaller than the lowest inter cluster divergence between cluster I and cluster IV
(91.948), thus validating the clustering pattern formed in this study. Cluster VI showed maximum intra cluster
distance (54.519) followed by cluster IV (30.323) and cluster II (38.535). Cluster V showed no intra cluster
distance because of having solitary genotype. Minimum and maximum inter cluster D
2
values referred close
relationship and maximum divergence between clusters, respectively. The maximum inter cluster distance was
observed between cluster II and cluster V (532.214) followed by cluster IV and cluster V (470.108), and cluster II
and cluster VI (407.136) (Table 1). Therefore, the genotypes falling in these clusters were genetically divergent
and suitable for chili hybridization programme to gain maximum heterosis.
Table 1 Average intra (bold) and inter cluster distance (D
2
) of chili genotypes with clustering pattern.
Cluster I
II
III
IV
V
VI
Genotype umber
Name of the genotype
I
33.803
144.110 156.547 91.948
405.033 283.864
9
G1, G2, G3, G5, G10, G18, G20, G21, G26
II
38.535
275.144 98.607
532.214 407.136
5
G4, G9, G15, G17, G30
III
43.800
210.636
273.845 180.777
7
G6, G7, G8, G13, G19, G24, G28
IV
30.323
470.108 351.652
6
G11, G12, G14, G16, G22, G23
V
0.000
161.960
1
G25
VI
54.519
2
G27, G29
1.3 Trait contribution towards genetic divergence
The contribution of different traits in per cent towards genetic divergence was presented in Figure 2. Among the
twelve characters studied in this present investigation, the maximum contribution towards divergence was found
