Molecular Plant Breeding 2016, Vol.7, No.29, 1
-
12
1
Research Article
Open
Access
Parent Selection for Intercrossing in Chili (
Capsicum annuum
L.) through
Multivariate Genetic Divergence Analysis
Matin Akand
1
*
, Rokib Hasan
2
*
, Nazmul Alam
2
, Abul Bashar
2
,
M Kamal Hossain
2
, A K M Mahmudul Huque
2,3
1 Regional Spices Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh
2 Plant Breeding and Crop Improvement Laboratory, Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
3 Department of Molecular Biology, Division of Life Sciences, Hana Science Hall, Korea University, Seoul 02841, Republic of Korea (South)
*This author has contributed equally to this work
Corresponding author
Molecular Plant Breeding, 2016, Vol.7, No.29 doi
Received: 01 Jun., 2016
Accepted: 15 Jul., 2016
Published: 12 Aug., 2016
Copyright © 2016
Matin et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Matin A., Rokib H., Nazmul A., Abul B.
,
M Kamal H., and A K M M.H., 2016, Parent Selection for Intercrossing in Chili (
Capsicum annuum
L.) through
Multivariate Genetic Divergence Analysis, 7(29): 1-12 (doi
Abstract
Thirty chili (
Capsicum annuum
L.) genotypes were evaluated to screen out suitable parents for hybridization programme
through multivariate analysis. Genetic diversity in chili genotypes based on twelve characters was estimated using Mahalanobis’s D
2
statistics. Six different clusters were formed through non-hierarchical clustering. The cluster I had the maximum number (9) of
genotypes followed by cluster III and IV with 7 and 6 genotypes. Cluster V contained only one genotype. The highest inter-cluster
distance was observed between cluster II and V (532.214) and the lowest inter-cluster distance was observed between the cluster I
and IV (91.948). The results indicated that fruit diameter (16%) contributed maximum to the total divergence followed by plant
height (14.5%) and fruits/plant (12.5%). Cluster VI produced highest mean for fruit diameter (14.04), fruits/plant (219). Cluster V
produced highest mean for fruit length (8.15) and yield/plant (617.13). Six different homozygous divergent parents G1, G11, G13,
G25, G17 and G30 were selected from six different clusters using variance ranking among genotypes within cluster. A Genotype by
trait (GT) biplot analysis was done to show the six potential parents from six different clusters along with their suitable characters in
one frame.
Keywords
Chili; Mahalanobis D
2
statistics; GT biplot
Introduction
Chili (
Caspsicum annum
L.) is the second most popular solanaceous vegetable after tomato. It is a diploid (2n =
24) species and genetically self-pollinated and chasmogamous plant (Lemma, 1998). Chili is believed to be
originated from South and Central America (Bahurupe et al., 2013). However, a rich genetic diversity of
Capsicum
exists due to varied geoclimatic conditions of Indian continent (Thul et al., 2009).
Chili, due to its pungent characteristic, has become a vital part of culinary cultures worldwide and has a long
history of use for flavoring, coloring, and preserving food. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide),
the major pungent bioactivator in the chili, is a homovanillic acid derivative that has been used medicinally for
centuries, but recently more extensively researches have been carried out for its analgesic (Simone et al., 1989 and
Brederson et al., 2013), antioxidant (Galano and Martinez, 2012), anti-inflammatory (Kim et al., 2003) and
anti-obesity (Kang et al., 2007) properties. The receptor for capsaicin is called the transient receptor potential
vanilloid subtype 1 (TRPV1) which belongs to the transient receptor potential (TRP) family. This family is a
heterogeneous group of non-selective cation channels (Sun et al., 2016). TRPV1 has prominent roles in oxidative
stress, inflammation and pain sensation (Mózsik, 2014). Recent findings have shown that TRPV1 plays a very
important role in the progression of cardiac hypertrophy, and thus, presents a possible
therapeutic target for the treatment of cardiac hypertrophy and heart failure (Buckley and Stokes, 2011). TRPV1
activation increases kidney function by enhancing glomerular filtration rate (Li and Wang, 2008), attenuates
abnormal glucose homeostasis by increasing insulin secretion and glucagon-like peptide 1 levels (Gram et al.,
2007; Wang et al., 2012), lowers blood pressure by promoting endothelium-dependent vasodilation (Yang et al.,
