Molecular Plant Breeding 2016, Vol.7, No.27, 1
-
9
1
Research Article Open Access
Phylogenetic Relationship of Lilies (
Lilium
) Analyzed based on trnH-psbA Barcode
Technology
Cui Jinteng
1,2,3
, Yang Xuezhen
1
, Zhang Kezhong
1,2,3
, Jia Yuehui
4
1 College of Landscape, Beijing University of Agriculture, 102206, Beijing, China
2 Beijing laboratory of Urban and rural ecological environment, 102206, Beijing, China
3 Beijing Engineering Research Center of rural landscape planning and design, 102206, Beijing, China
4 College of Foerstry and Horticulture, Beijing University of Agriculture, 102206, Beijing, China
Corresponding author Email
Molecular Plant Breeding, 2016, Vol.7, No.27 doi
Received: 4 Jun., 2016
Accepted: 10 Jun., 2016
Published: 24 Jun., 2016
Copyright © 2016
Cui et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Cui J.T., Yang X.Z., Zhang K.Z., and Jia Y.H., 2016, Phylogenetic Relationship of Lilies (
Lilium
) Analyzed based on trnH-psbA Barcode Technology,
Molecular Plant Breeding, 7(27): 1-9 (doi
Abstract
Phylogenetic relationship of
lilium
plants was studied by means of trnH-psbA DNA barcode technique. The result
demonstrated that
trnH-psbA
sequence length varied in different
lilium
plants. The entire length of
trnH-psbA
sequence was 460 bp
after multiple alignments. The number of conserved sites was 415 and that of variable sites was 45. A total of 63 tested materials
were clustered into five groups.
Lilium davidii
, 7 Asiatic cultivars, one cross progeny between Asiatic cultivar and wild parental
species, were clustered into one subgroup (the
Ⅰ
a subgroups).
Lilium speciosum
var. gloriosoides, 10 Oriental cultivars and 2
intra-section Archelirion cross progenes were gathered into one subgroup (the
Ⅰ
b subgroup ). Majority of the tested wild species
were clustered into the
Ⅱ
group which showed the characteristics of intra-species and geography. To some extent, the phylogenetic
relationship of wild lilies in
Ⅱ
group via
trnH-psbA
barcode was inconsistent with the result obtained from traditional
morphological classification method. The other 4 Asiatic cultivars and one cross progeny between one Asiatic cultivar and wild
parental species were clustered into the
Ⅲ
group. Therefore, the Asiatic cultivars in
Ⅰ
b subgroup and those in the
Ⅲ
group were
originated from different lilies in the section
Sinomartagon
.
Keywords
Lilium brownie
, Phylogenetic relationship,
trnH-psbA
barcode
Introduction
Lily has an unusual richness of germplasm resources which about 8 000 cultivars have been registered (Gu, 2013).
To study their phylogenetic relationship could be helpful to reveal the relationship among interspecies,
intraspecies and cultivars; to find new species or unclear (obscure) species would facilitate to building the basis
for parent selection in hybrid breeding program as well. Morphological markers (Li, 2010) and cytological
markers (Peng, 2012) were used to mine the phylogenetic relationship among species, yet phenotypic diversity is
not always able to present the real diversity in essence of hereditary because the morphological characteristics
should be the results generated by combined actions between genetic effect and external environment factors. The
molecular genetic markers are rarely used in the research of lily phylogenetic relationship due to the limited
numbers of the markers. In recent years, RAPD (Li, 2013), SRAP (Zhen, 2010), SSR (Yu, 2013), ISSR (Wu, 2010)
and other molecular markers have been used to study the phylogenetic relationship of lilies. RAPD amplification
is poor reproducible due to its short length of primer; SRAP is distributed randomly and irregularly on the
chromosome due to the molecular marker failed to use of the amplification sequence, and the marker is applied
with high cost; screening and identifying the SRAP needs heavy workload, but the efficiency is pretty low as well.
The amplification by ISSR has better stability that of RAPD due to its longer length of primer. The three kinds of
molecular markers mentioned above would be able to achieve the good results in analysis of intraspecific
relationship, but limitations applied in analysis of interspecies.
DNA barcoding is a molecular biological technique using DNA sequence information to identify species or their
variant types. It mainly uses a group of short homologous DNA sequences coming from different biological
