Molecular Plant Breeding 2016, Vol.7, No.27, 1
-
9
5
subgroups were the source of Northeast Jilin.
Five lily samples below the second category in the phylogenetic tree were classified as the third category
(Category III)lass (
Ⅲ
), 'Gironde' No.44,
‘
Val di sole
’
No.59,
‘
Madras
’
No.61,
‘
Navona
’×
Lilium dauricum
No.64 and
‘
Renoir
’
No.58. Among these five samples, four of them were Asian lilies except (
‘
Navona
’×
Lilium dauricum) No.64 was hybrid offspring derived fromAsian lily and one of its primitive parents.
The two lily materials were clustered into the fourth class (
Ⅳ
) below the third class in the clustering figure, they
were No.20 Hubebaihe (Xuanen county Wanzhai township, section sinomatagon), No.3 Yebaihe (Shenlongjia
songluo town, Section Leucolirion), both of them were coming from Hubei province.
On the bottom of phylogenetic tree, there were 8 wild lilies, clustering into the fifth classes (Class V), which can
be further divided into 2 subclasses. The first subclass (
Ⅴ
a) was composed by four samples: No.12 Danhuabaihe
(Shenlongjia Hongping town, section leucolirion), No.1
Lily
(Xuanen county Wanzhai township, section
leucolirion), No.32 Juandan (Taibai county Taochuan town, Sect. Sinomartagon), and No.8 Juandan
(Fengchen
county Baoshan town, Sect. Sinomartagon), which includes both section lilium and section sinomatagon (e.g.
No.32 and No.8).
The second subclass (
Ⅴ
b) all came from Northwest Yunnan, including No.23 Huanglv huadian (Dali city
Cangshan wutaifeng, generalized section sinomatagon), No.22 Dalibaihe
(Binchuan county Laishan mountain,
section sinomatagon), No.24 Dianbaihe (Lijiang Yulongxueshan mountain, generalized section sinomatagon), and
No.14 Huangjinhua Dianbaihe (Haidong county Daqingshan mountain, generalized section sinomatagon).
2 Discussion
2.1 Comparison between traditional classification method and the
trnH-psbA
barcode technology in lily
phylogenetics
When we use traditional morphological classification method to do the phylogenetic classification for original
Lily species, flower type is the first classification criteria in Chinese morphological taxonomy method. They will
be divided into four groups: section lilium, section lophophorum, section sinomatagon and section martagon. This
classification method will merge some wild original parents of Asian cultivar (such as
L. speciosum
and its
variants
L. speciosum
var.
gloriosoides
) into Section sinomatagon, and couldn’t explain the fact of hybrid
difficulty and distant genetic relationship between Asian cultivar and Asian Lily (originated in Section
sinomatagon). While the national morphological taxonomy usually take leaf type as the first classification criteria,
flower type as the second classification criteria, combined with epigaeous, bulb color and shape divided original
Lily species into seven groups. The
L. speciosum
,
L. speciosum
var.
gloriosoides
are segmented into section
archelirion instead of section sinomatagon which provide a well explaination for the fact of distant genetic
relationship between Oriental cultivar and Asiatic Lily. Therefore, there obviously are limitations in Chinese
traditional morphological methods in Lily classification, and the classification of Lily into seven groups according
international system is now generally used.
In our study, the second category (
Ⅱ
) included 4 sections of wild lilies, i.e. section of Sinomartagon, section of
Leucolirion, section of Daurolirion and section of Martagon. Although Danhuanghua Bahe (Lilium
sulphureum)(germplasm origin from Sanying Town, Eryuan County) and Yichang Bahe (Lilium leucanthum )
(germplasm origin from Chadianzi, Badong County) should be grouped in the section of Leucolirion, the evidence
presented that they can’t get together, which might be due to these wild lilies came from different geographic
regions and different ecological environments where exist much more geographical isolation and reproductive
barrier, resulting in the evolution of the chloroplast genes along different ways.
In addition, in the fourth category (Category
Ⅳ
), Hubei Baihe (Lilium henryi) belongs to section of
Sinomartagon and wild lilies belongs to section of Leucolirion. According to the traditional taxonomic to analysis
hybridization between the two groups is very diffcult. But it has been reported that Hubei Baihe (Lilium henryi)
