Molecular Plant Breeding 2016, Vol.7, No.26, 1
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Table 2 Primer combinations and polymorphism from DNA sequence of Celosia
Primer
combination
Number of fragments
amplified
Number
of
Polymorphic bands
Gene
diversity
Total number
of bands
Polymorphic information
content (PIC)
DNA
standard size
AAC+CAG 40
156
0.9
400
0.8910*
100-base pair
(bp)
AAC+CAA 39
94
0.9
390
0.8910*
100-base pair
(bp)
AAC+CAC
38
140
0.9
380
0.8910*
100-base pair
(bp)
Mean
39
130
0.9
390
0.891
The dendrogram result showed the delineation of ten genotypes of
C. argentea
into three cluster groups (Figure 1).
The cluster group 1 comprises of genotypes NGB/01260, NG/MA/MAY/09/015, NHGB/09/160 and
NIHORT/0001, in which NGB/01260 and NG/MA/MAY/09/015 as well as NHGB/09/160 and NIHORT/0001 are
more genetically related to each other. Also, the second cluster group comprises of NG/MAY/09/015 and
NG/SA/07/213, while NHGB/01260, NG/AO/MAY/09/015, NG/MR/MAY/09/015 and NG/TO/MAY/09/015
genotypes constituted the third cluster group. Genotypes NHGB/01260 and NG/AO/MAY/09/015 as well as
NG/MR/MAY/09/015 and NG/TO/MAY/09/015 are also genetically related to each other.
Figure 1 Dendrogram tree showing the genetic relationship among genotypes of
Celosia argentea
The result from Figure 2 showed the optimization reactions of genomic DNA of
C. argentea
genotypes carried out
in four reactions using six different primer combinations. Reaction 3 produced the best scorable amplified bands
followed by reaction 1. In reaction 1, G1, G4, G5 and G10 revealed the most noticeable bands compared to other
genotypes.
Figure 2 Agarose gel profile showing the optimization of genomic DNA of
Celosia
argentea
genotypes
