Molecular Plant Breeding 2016, Vol.7, No.26, 1
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Research Report Open Access
Molecular Variability of
Celosia argentea
Using Amplified Fragment Length
Polymorphism (AFLP) Marker
Bamigbegbin B.J. , Olawuyi O.J., Jonathan S.G.
Genetics and Molecular Biology Unit, Department of Botany, University of Ibadan, Nigeria
Corresponding author Email
Molecular Plant Breeding, 2016, Vol.7, No.26 doi
Received: 14 Oct., 2015
Accepted: 25 Nov., 2015
Published: 12 Jun., 2016
Copyright © 2016
Bamigbegbin et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Bamigbegbin B.J., Olawuyi O.J., and Jonathan S.G., 2016, Molecular variability of
Celosia argentea
using amplified fragment length polymorphism (AFLP)
marker, Molecular Plant Breeding, 7(26): 1-6 (doi
Abstract
The molecular variability of ten genotypes of
Celosia argentea
seeds collected from National Institute of Horticultural Research
(NIHORT) and National Centre for Genetic Resources and Biotechnology (NACGRAB) germplasms were evaluated using the Amplified Fragment
Length Polymorphism (AFLP) marker. The polymorphism of
C. argentea
was detected within the population using primer mix of AFLP EcoRI +
MseI adaptors type in three primer combinations. Powermarker software V3.25 was statistically used to analyse the fragments from extracted DNA
region. The highest concentrations of genomic DNA of 13.30 μL and volume of 2 217.59 μL for total genomic DNA were recorded for
NG/TO/MAY/09/015 and NG/MA/MAY/09/015 genotypes respectively. Variations were observed in the number of fragments amplified by each of
the three AFLP primers combinations. The polymorphic information content (PIC) of the amplified fragment of the genomic DNA was diverse at
89.1% for DNA size of 100 base pairs, while the percentage gene diversity was 90%. The primer sequence combination of AAC + CAG produced
the highest number of bands, amplified fragments, and number of polymorphic bands of 400, 40, and 156.000 respectively. A dendrogram
constructed revealed three cluster groups, in which clusters 1 and 3 were delineated into 4 genotypes each, while cluster 2 had the least with two
genotypes. This study revealed variability among the genome of
C. argentea
using AFLPmarker. This could promote improvement and conservation
of
C. argentea
germplasm for broaden genetic basis of breeding program.
Keywords
Celosia argentea;
Amplified fragment length polymorphism (AFLP); Genotypes; Dendrogram
Introduction
Celosia argentea
L. is a widely cultivated vegetable crop in Amaranthaceae family as traditional food (Denton,
2004). It is commonly known as Lagos spinach or sokoyokota in Yoruba that is, “make husbands fat and happy”
(Grubben and Denton, 2004). Young leaves of
C. argentea
harvested 5-7 weeks after sowing have the best
nutritional value; it contains water, energy, protein, fat, carbohydrate, fibre, Calcium, Phosphorus, Iron, Vitamins
A and C with high content of micronutrients comparable with some vegetables. Again, the leaves contain phytic
acid and oxalic acid which makes it less suitable for fresh consumption (Ayodele and Olajide, 2011). The
composition is strongly influenced by environmental factors which include soil fertility, fertilizer application and
age of plant at harvest (Denton, 2004). The genetic variability of this crop had been characterized phenotypically
with high significant variation detected in this population (Olawuyi et al., 2016). The need for genetic base and
distinctive useful traits which may be eroded in crop adaptation, improvement, and biodiversity conservation
necessitated this study. Hence, there is need for molecular evaluation of
C. argentea
to validate it. Polymerase
Chain Reaction (PCR) of many molecular techniques has been developed for plant genomic analysis (Esayas and
Bryngelsson, 2006). They are more reliable for genetic studies than morphological characteristics, time efficient
and cover the whole organism’s genome. Molecular techniques made it possible to isolate and analyze specific
genes, enhance the understanding of organism’s genome, generate genetic maps and advancement of gene therapy
technologies. Moreover, they have played crucial roles in phylogeny studies and species evolution using DNA
sequence in manipulation of genetic variation within populations. Recently, the use of Amplified Fragment Length
Polymorphism (AFLP) marker has become the main tool for disclosing a high number of polymorphic markers by
single reaction (Vos et al., 1995). It is a useful technique for breeders to facilitate plant improvement, using
molecular genetics maps to undertake marker-assisted selection and positional cloning for some characters. The
