Molecular Plant Breeding 2016, Vol.7, No.26, 1
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identification of genetic relationship among the cultivars based on biochemical and molecular analysis is essential
in genetic improvement and selection of crossing combinations from bulk parental genotypes. AFLP markers have
successfully been used for analyzing genetic diversity in some species of Amaranthaceae such as Brazilian
Ginseng (Figueira et al., 2011), Palmer Amaranths (Aman et al., 2013) and Weed Amaranths (Wassom and Tranel,
2005). However, there is limited information on molecular variability of Celosia using AFLP marker. This study
therefore aimed at investigating variability of
C. argentea
using molecular technique.
1 Results, Recommendations and Suggestions
The AFLP molecular marker revealed genetic variability among the
C. argentea
genotypes based on the number
of amplified fragments with polymorphic information content of 89.1% and gene diversity of 90%. The variability
of morphological and yield traits in the breeding materials have their roots in the organism’s genome. This will
ensure germplasm conservation and development of strategies in improvement of
C. argentea
. The highest
concentrations of genomic DNA of 13.30
μL
and volume of 2 217.59
μL
for total genomic DNA were recorded for
NG/TO/MAY/09/015 and NG/MA/MAY/09/015 respectively. These genotypes could be recommended for crop
improvement breeding program. Hybridization breeding of these genotypes is suggested for its crop improvement
with desired traits in the parent lines. Also, primer sequence and compatibility are seemly unending challenges in
molecular studies, therefore; the primer combinations in its compatibility for the crop studied could be
documented and recommended for pilot study in molecular evaluation of other vegetable crops.
2 Discussion
The nano drop DNA quantization for the extracted
C. argentea
genotypes was found at 260/280 ng/μL (Table 1).
The quality of genomic DNA concentration for NG/TO/MAY/09/015 had the highest value of 13.30 μL, while the
volume of total genomic DNA was 376.12 μL. Though, genotype NG/MA/MAY/09/015 produced the highest
quantity, but lesser quality, while the lowest quality of genomic DNA at 2.08 μL from total volume of 464.70 μL
was recorded for NHGB/09/160.
Table 1 DNA concentration extracted from ten genotypes of
Celosia argentea
SN
Genotypes
Total volume of extracted genomic DNA (μ/L)
DNA concentration (μ/L)
1
NHGB/01260
451.61
11.1
2
NG/MR/MAY/09/015
507.32
9.9
3
NG/TO/MAY/09/015
376.12
13.3
4
NG/SA/07/213
808.74
6.2
5
NG/MA/MAY/09/015
2217.59
2.3
6
NHGB/09/160
464.7
2.08
7
NIHORT/0001
1002.85
5
8
NG/MAY/09/015
397.93
12.6
9
NG/SA/07/213
1179.32
4.2
10
NG/AO/MAY/09/015
904.1
5.5
Mean
831.00*
7.22*
The polymorphic information content and amplification patterns of the genome of
C. argentea
are shown in Table
2. The percentage gene diversity recorded was 90%, while the polymorphism in the population was diverse at
89.1% for standardized DNA size of 100 base pairs. There are variations in number of amplified fragments,
number of polymorphic bands and total number of bands. Primer sequence combination of AAC + CAG produced
the highest number of bands, amplified fragments and number of polymorphic bands of 400, 40, and 156.000 0
respectively.
