Molecular Plant Breeding 2016, Vol.7, No.14, 1
-
10
4
sub-genome of (AD)
2
.
Figure 2 GISH of (AD)
1
with all 13 D genome species as probes (all probes were labeled with digoxigenin, and red fluorescence
signals were observed on D sub-genome chromosomes in each Fig). Mitotic metaphase chromosomes of (AD)
1
with
G. thurberi
(D
1
)
(a),
G. armourianum
(D
2-1
) (b),
G. harknessii
(D
2-2
) (c),
G. klotzschianum
(D
3-k
) (d),
G. davidsonii
(D
3-d
) (e),
G. aridum
(D
4
) (f),
G.
raimondii
(D
5
) (g),
G. gossypiodies
(D
6
) (h),
G. lobatum
(D
7
) (i),
G. trilobum
(D
8
) (j),
G. laxum
(D
9
) (k),
G. turneri,
(D
10
) (l),
G.
schwendimanii
(D
11
) (m) gDNA as probe respectively, blocking with ssDNA (In each Fig, there are six arrows, all arrows show
signals of GISH-NORs, of which two green arrows denote the GISH-NORs in A sub-genome and four white arrows denote the
GISH-NORs in D sub-genome). Bars=10
m.
DA values of (AD)
1
generated by all 13 diploid D gDNA probes respectively were shown in Table 2. Because of
the hybridization signals generated by D
6
gDNA probe were distributed on both A and D sub-genome
chromosomes of (AD)
1
, therefore the DA value generated by D
6
gDNA probe(DA value was 0.402) was much
higher than the other 12 D gDNA probes in (AD)
1
. Except the D
6
gDNA probe, the highest DA value was 0.286
generated by D
3-d
gDNA probe, and then followed by D
4
, D
1
, D
5,
D
11
, D
2-1
, D
2-2
, D
8
, D
3-k
, D
9
, D
10
and D
7
probes.
This showed that the D
3-d
gDNA probe possess the strongest ability to recognize the D sub-genome chromosomes
