Molecular Plant Breeding 2016, Vol.7, No.14, 1
-
10
1
Research Article Open Access
Analyses on Progenitor Donors of the Cultivated Allotetraploid Cottons
Revealed by GISH
Xiao Shuiping
1,2
, Wang Kunbo
2
, Yang Lei
1
, Ke Xingsheng
1
, Wang Chunying
2
, Liu Xinwen
1
, Sun Liangqing
1
, Yang Shaoqun
1
, Liu
Fang
2
, Chen Yi
1
1 Cotton Research Institute of Jiangxi Province/ Poyang Lake Cotton Experiment Station, CARS, Jiujiang, Jiangxi 332105, China
2 Institute of Cotton Research of CAAS / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
Corresponding authors Email:
, liufcri @163.com
Molecular Plant Breeding, 2016, Vol.7, No.14 doi
Received: 12 Jan., 2016
Accepted: 27 Feb., 2016
Published: 07 Apr., 2016
Copyright © 2015
Xiao et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Xiao S.P., Wang K.B., Yang L., Ke X.S., Wang C.Y., Liu X.W., Sun L.Q., Yang S.Q., Liu F., and Chen Y., 2016, Analyses on Progenitor Donors of the
Cultivated Allotetraploid Cottons Revealed by GISH, Molecular Plant Breeding, 7(14): 1-10 (doi
Abstract
GISH (Genomic i
n situ
hybridization) of the mitotic metaphase chromosomes
of two cultivated tetraploid cotton
(AD)
1
(
G.
hirsutum
) and (AD)
2
(
G. barbadense
) with all 3 diploid A cotton gDNA(genomic DNA) as probes, blocking with ssDNA(salmon
sperm DNA) respectively. The hybridization signals were dected distribute in the A sub-genome chromosomes of (AD)
1
and (AD)
2
,
besides, three pairs of crimson signals were also detected only with the A
1-a
gDNA probe which named GISH-NORs. GISH of (AD)
1
and (AD)
2
with all 13 diploid D cotton gDNA as probes, blocking with ssDNA respectively, except the D
6
(
G.gossypiodies
) gDNA
probe generated the hybridization signals in all the chromosomes of (AD)
1
and (AD)
2
, the other 12 diploid D gDNA probes only
generated the signals on the D sub-genome chromosomes of (AD)
1
and (AD)
2
, the D6 gDNA probe was very specifical.And three
pairs of strong GISH-NORs were detected with all 13 diploid D genome species gDNA probes, the intensity of their GISH-NORs
were much brighter than the A
1-a
gDNA probe. These results visually confirmed the amphidiploid origin of the allotetraploid cotton
species. DA (distinguishing ability ) values of each gDNA probe generated were calculated basing on the above GISH rsults.It
showed that the DA value of A
1-a
gDNA probe was the biggest in all 3 diploid A genomes both in (AD)
1
and (AD)
2
GISH, and this
indicated that A
1-a
genome was most likely to be the A sub-genome progenitor donor of (AD)
1
and (AD)
2
, while the D
3-d
(
G.
davidsonii
) and D
5
(
G. raimondii
) genome species were most likely to be the D sub-genome progenitor donor of (AD)
1
, and (AD)
2
respectively. And this further confirmed that tetraploid cottons are polyphyletic.
Keywords
Gossypium; G.hirsutum
L.;
G.barbadense
L.; Progenitor; GISH; GISH-NOR
Introduction
Gossypium
genus belongs to the Malvaceae family, which contains 46 diploid (2n = 2× = 26,) species and 5
tetraploid (2n = 4× = 52). The
Gossypium
genus are believed to have originated from a common ancestor
approximately
5～
10 million years ago
，
of which eight diploid genomes, designated as A to G and K, have been
found across Africa, Asia Australia and North America
(
Phillips,1963; Wendel ,1989; Fryxell,1992; Dejooded and
Wendel, 1992), and 5 tetraploid species were named (AD)
1
(
G. hirsutum
), (AD)
2
(
G. barbadense
), (AD)
3
(
G.
tomentosum
), (AD)
4
(
G. mustelinum
) and (AD)
5
(
G. darwinii
) respectively(Wendel ,1989). At present, (AD)
1
and
(AD)
2
are major cultivated species, which are extensive cultivated in the world. Previous studies had shown that
diploid cotton A and D genome species are the respective donor species of A and D sub-genome of the
allotetraploid cotton species, and the A sub-genome chromosomes are longer than the D sub-genome
chromosomes(Phillips,1966; Endrizzi,1985).
The Fluorescence
in situ
hybridization (FISH) technology was introduced to plant research in 1985
(Rayburn,1985), which has applied to various aspects such as repeat sequences positioning, chromosome
identification, cell genetic map construction, the origin of polyploid genome evolution and phylogenetic
relationships and so on (Snowdon et al., 1997 ; Tang et al., 2000; Jiang and Gill,2006; Wang k et al.,2008; Nemeth
et al., 2015; Melo et al., 2015).
