Molecular Plant Breeding 2016, Vol.7, No.14, 1
-
10
5
of (AD)
1
, while the other D gDNA probes show weaker ability to recognize the D sub-genome chromosomes of
(AD)
1
, therefore the D
3-d
genome had closer genetic relationship with the D sub-genome of (AD)
1
.
Figure 3 GISH of (AD)
2
with all 13 D genome species as probes (all probes were labeled with digoxigenin, and red fluorescence
signals were observed on D sub-genome chromosomes in each Fig, all generated GISH-NORs). Mitotic metaphase chromosomes
of (AD)
2
with
G. thurberi
(D
1
) (a),
G. armourianum
(D
2-1
) (b),
G. harknessii
(D
2-2
) (c),
G. klotzschianum
(D
3-k
) (d),
G. davidsonii
(D
3-d
) (e),
G. aridum
(D
4
) (f),
G. raimondii
(D
5
) (g),
G. gossypiodies
(D
6
) (h),
G. lobatum
(D
7
) (i),
G. trilobum
(D
8
) (j),
G. laxum
(D
9
)
(k),
G. turneri,
(D
10
) (l),
G. schwendimanii
(D
11
) (m) gDNA as probe respectively, blocking with ssDNA (In each Fig, there are six
arrows, all arrows show the GISH-NORs, of which two green arrows denote the GISH-NORs in A sub-genome while other four
white arrows denote the GISH-NORs in D sub-genome). Bars=10
m.
DA values of (AD)
2
generated by all 13diploid D gDNA probes also be shown in Table 2. Except the D
6
gDNA
probe(DA value was 0.387), the highest DA value generated by D
5
gDNA probe was 0.263, and then followed by
D
3-d
, D
1
, D
4
, D
2-1
, D
2-2
, D
11
, D
8
, D
3-k
, D
10
, D
9
and D
7
probes. which was also higher than those of the other 12 D
gDNA probes. The results showed that the D
5
gDNA possess the strongest ability to recognize the D sub-genome
