International Journal of Aquaculture, 2016, Vol.6, No.6, 1
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10
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Oocytes are about 425 µm to 439 µm in diameter. The nucleus becomes larger with diameter from 75 µm to 170 µm,
zona radiata with mean thickness of 16 µm (Figure c).
Figure 2 Cross section of ovary showing
Immature &
Recovering stage
of
Liza carinata
stained with
eosin and
hematoxilin
(10 X)
Figure c Cross section of ovaries of
Liza carinata
showing
Vacuolization Stage
stained with
eosin and hematoxilin
(40X)
Note:
(
N) nucleus, (NU) nucleoli, (CM) chromatine material,
(YV) yolk vesicles, (V) vacuoles, (FE) follicular epithelium
Presence of yolk vesicle in the ovary indicated that the
Developing stage
is started and this is observed throughout the
investigation months especially in September and October (Figure 3).
2 Yolk stages:
(i)
Primary yolk stage:
Oocyte become greater ranged from 576 µm to 670 µm in diameter, characterized by presence of yolk vesicle layers
and nucleus mean diameter became 173 µm zona radiata increases in thickness to be 31.9 µm (Figure d
1
).
Figure 3 Cross section of ovary showing
Developing stage
of
Liza carinata
stained with
eosin and hematoxilin
(10 X)
Figure d
1
Cross section of ovaries of
Liza carinata
showing
Primary Yolk Stage
stained with
eosin and hematoxilin
(40X)
Note:
(
N) nucleus, (NU) nucleoli, (CM) chromatine material,
(YV) yolk vesicles, (V) vacuoles, (FE) follicular epithelium,
(ZR) zona radiata
(ii)
Secondary yolk stage:
Oocyte become much larger with diameter ranged from 646 µm to 715 µm, characterized by presence of yolk globules,
and irregularly nucleus shape with average diameter 123 µm zona radiata increases in width to an average of 30 µm
(Figure d
2
).
(iii)
Tertiary yolk stage:
The diameter of oocyte generally greater than 650 µm and less than 766 µm with presence of yolk globules in much
size and number, the nucleus mean diameter became 88 µm and zona radiata varies in thickness with average about 39
