IJA-2016v6n6 - page 8

International Journal of Aquaculture, 2016, Vol.6, No.6, 1
-
10
3
Oogonia are spherical with diameter 44.5 µm to less than 45.5 µm, having large nucleous with diameter from 10 µm to
11 µm. (Figure a) ovary which fill of oogonia represented the
Immature stage
which found from February till August
(Figure 1).
Figure a Cross section of ovaries of
Liza carinata
showing
Chromatin-Nucleolus stage
stained with
eosin and
hematoxilin
(100X)
,
arrow showing oogonia
Figure 1 Cross section of ovary showing
Immature stage
of
Liza carinata
stained with
eosin and hematoxilin
(10X)
2 Perinucleolus stage:
(i)
Early Perinucleolus stage:
Oocyte rounded with diameter ranged from 22.5 µm to less than 38 µm. The nucleus with diameter ranged from 16 µm
to 28 µm (Figure b
1
).
(ii)
Late Perinucleolus stage:
Oocyte divided into two sizes, the smallest size ranged from 92 µm to 100 µm in diameter with nucleus ranged from 55
µm to 70 µm in diameter.
The diameter of the largest oocyte ranged from 101 µm to 116 µm with nucleus ranged from 60 µm to 70 µm in
diameter (Figure b
2
).
Figure b
1
Cross section of ovaries of
Liza carinata
showing
Early Perinucleolus Stage
stained with
eosin and hematoxilin
(100X)
Note: A showing early Perinucleolus Stage and B showing late
Perinucleolus Stage
Figure b
2
Cross section of ovaries of
Liza carinata
left arrow
showing
Late Perinucleolus Stage
stained with
eosin and
hematoxilin
(40X)
Note: (N) nucleus, (NU) nucleoli, (CM) chromatine material,
(FE) follicular epithelium, right arrow showing yolk nucleous
Ovary with early and late prinucleolus stage found at the end of spawning season from March to September to represent
Immature & Recovering stage
(Figure 2).
II – Second growth phase
1 Vacuolization stage (yolk vesicle stage):
1,2,3,4,5,6,7 9,10,11,12,13,14,15,16
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