International Journal of Aquaculture, 2016, Vol.6, No.6, 1
-
10
5
µm in width (Figure d
3
).
Figure d
2
Cross section of ovaries of
Liza carinata
showing
Secondary Yolk Stage
stained with
eosin and hematoxilin
(40X)
Note: (N) nucleus, (NU) nucleoli, (CM) chromatine material,
(YV) yolk vesicles, (YG) yolk globule, (V) vacuoles, (FE)
Follicular epithelium, (ZR) zona radiata
Figure d
3
Cross section of ovaries of
Liza carinata
showing
Tertiary Yolk Stage
stained with
eosin and hematoxilin
(40X)
Note: (N) nucleus, (NU) nucleoli, (CM) chromatine material,
(YG) yolk globule, (V) vacuoles, (FE) follicular epithelium,
(ZR) zona radiata
The presence of oocytes from all yolky stages of development (primary, secondary and tertiary yolk globule) in the
ovary found from October to November and showed the
Maturing stage
of ovary (Figure 4).
3 Ripe stage:
The egg are approximately from 925 µm to 933 µm in diameter and yolk appears to fill the interior of the oocyte, the
nucleous migrated to the animal pole, zona radiata varied in thickness from about 22.5 µm at the vegetative pole to 36
µm at the animal pole (Figure e).
Figure 4 Cross section of ovary showing
Maturing stage
of
Liza carinata
stained with
eosin and hematoxilin
(10X)
Figure e Cross section of ovaries of
Liza carinata
showing
Ripe Stage
of oocyte stained with
eosin and hematoxilin
(40X)
Note: (YG) yolk globule, (V) vacuoles, (FE) follicular
epithelium, (ZR) zona radiata
Ovary with ripe eggs represented the
Mature stage
and this stage found from November to December (Figure 5).
4 Spawning stage:
Ripe or mature ova are spawning, the ovarian membrane is thin and wrinkled than the normal one and the ovarian
cavity appears large. This stage found from November to March (Figure 6).
5 Spent stage:
Spent ovary contain empty and rupture follicles at different phases some of them at resting phase representing few
oogonia, many perinucleolus oocyte and atretic oocytes (oocytes can’t be spawned due to unfavorable conditions) and
