International Journal of Aquaculture, 2016, Vol.6, No.2, 1
-
9
7
medium doses (quadratic effect) was described by several authors in aquaculture (Bagheri et al., 2008; Faramarzi
et al., 2011; Kapareiko et al., 2011).
Statistical evaluations of the results of administering BAL suspension did not show significant differences. None
of the treatments with dose 4, regardless stage of administration, showed values of survival, mean weight and
biomass higher than the control group. Then, we selected dose 4 as the most adequate to be incorporated in future
assays. This result, although not expected, support the concept that a higher number of probiotic cells included in
diets and host intestine does not necessarily result in a highest or improved growth, survival and protein efficiency
ratio of animals (Bagheri et al., 2008).
The macroscopic observations of the animals did not show modifications in their behavior and/or development of
those treated with the probiotic strains when compared with control. The histological evaluation demonstrated no
translocation or aggrupation of microorganisms, studies that needs to be performed to determine the safety of the
strains used as probiotics (Pasteris et al., 2009; ISAPP, 2011). Histological studies of major organs and tissues
were comparable to those described as normal for each stage (Mendoza et al., 2013). The results obtained indicate
that the administration of the two formulae in the doses herein evaluated did not affect the normal development
and function of animals.
Then, the dose 7 (6x10
7
CFU L-1 of each strain) of the ENT suspension (composed by strains
E. faecium
CRL
1937 and
E. faecium
CRL 1938) and dose 4 (6x10
4
CFU L-1 of each strain) of the LAB suspension (composed by
strains
E. faecium
CRL 1940 and CRL 1941 and
P.acidilactici
CRL 1939) were selected to be included in a
potentially probiotic mixture to be assayed
in vivo
in future assays. And a combination of these strains with three
autochthonous
Bacillus subtilis
strains, were registered for patent aims (Conicet and Unne, 2013).
Acknowledgments
The Argentinian National Council of Scientific and Technical Researches (Consejo Nacional de Investigaciones Científicas y
Técnicas–CONICET), projects numbers PIP 632 and PIP 744, together with the National Agency for Scientific and Technological
Promotion (Agencia Nacional de Promoción Científica y Tecnológica–ANPCyT) through the Fund for Scientific and Technological
Research (Fondo para la Investigación Científica y Tecnológica–FONCyT); projects numbers PICTO-UNNE-2007-161,
PICTO-UNNE-2011-198, PICT-543, PICT-1088 and PICT-1187; financed this study. Some of the results of this paper were included
in a patent presentation in Argentina (INPI Exp 20130103820)
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