International Journal of Aquaculture, 2016, Vol.6, No.15, 1
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fed the control diet (Table 4).The mean final albumin and globulin followed the same trend as the total protein in
the fish fed the experimental diets (Table 4).
Table 3 Haematological parameters of
Clarias gariepinus
fed amoxicillin meal diets
Parameters
Initial
AXM1
AXM2
AXM3
Pack cell volume
19.00 ±0.58
d
22.33 ±0.88
b
27.67 ±1.45
a
26.67 ±1.89
a
Haemoglobin (g/dl)
6.30 ±0.17
b
7.40 ±0.45
ab
8.83 ±0.55
a
8.33 ±0.44
a
Red blood cell count x10
6
/dl
3.51 ±0.41
c
11.64 ±1.49
b
14.57 ±0.58
ab
17.47 ±1.57
a
White blood cell ( X10
3
/dl)
7.80 ±0.87
c
11.82 ±0.70
a
9.20 ±0.35
b
8.87 ±1.45
b
Platelet
8.00 ±1.15
b
11.00 ±0.58
a
11.53 ±0.88
a
8.33 ±0.33
b
Mean cell volume (x10
4
/l)
55.00 ±7.00
65.67 ±2.85
a
65.33 ±1.15
a
63.00 ±3.00
a
Mean cell haemoglobin (X10
-5
/l)
18.33 ±2.33
34.33 ±2.40
a
32.00 ±1.53
a
32.33 ±1.45
a
Mean cell haemoglobin concentration (g/100ml)
33.00 ±0.00
a
32.00 ±0.00
32.67 ±0.67
33.00 ±0.57
Lymphocytes (%)
62.67 ±4.06
69.83 ±2.89
75.67 ±2.33
63.33 ±6.01
Neutrophils (%)
37.33 ±4.06
32.50 ±1.80
34.00 ±2.08
37.83 ±4.34
Erythrocyte Sedimentation Rate
1.33 ±0.33
b
2.33 ±0.33
a
2.00 ±1.00
a
2.67 ±0.67
a
Note: Values with different superscripts in each row are significantly different (P > 0.05)
Table 4 Serum biochemical indices of
Clarias gariepinus
fed amoxicillin meal diets
Parameters
Initial
AMX1
AMX2
AMX3
Total protein (g/100 mls)
5.83 ±0.07
b
6.30 ±0.15
b
6.90 ±0.06
a
7.27 ±0.09
a
Albumin (g/100 mls)
1.80 ±0.06
b
2.15 ±0.25
b
2.70 ±0.17
a
3.07 ±0.03
a
Globulin (g/100 mls)
4.03 ±0.03
4.17 ±0.36
4.20 ±0.12
4.21 ±0.06
Albumin/Globulin ratio
0.44 ±0.01
0.41 ±0.08
b
0.65 ±0.06
a
0.73 ±0.01
a
Blood glucose (g/100 mls)
35.67 ±0.88
50.33 ±1.20
a
51.00 ±1.00
a
45.67 ±1.45
b
K (mmol/l)
29.33 ±14.85
56.67 ±6.01
c
76.67 ±0.88
b
96.33 ±3.48
a
Na (mmol/l)
60.67 ±30.38
91.33 ±1.86
95.33 ±2.91
94.33 ±3.48
Creatinine (mg/dl)
1.66 ±0.32
1.47 ±0.32
1.76 ±0.22
1.78 ±0.19
BUN (mg/dl)
2.67 ±0.15
2.83 ±0.12
2.73 ±0.07
2.97 ±0.03
AST (µl)
20.00 ±2.31
18.33 ±3.38
a
25.33 ±2.91
a
29.67 ±3.18
a
ALT (µl)
72.00 ±6.43
61.00 ±2.08
b
82.67 ±1.45
a
87.33 ±1.76
a
ALP (µl)
24.17 ±4.04
35.33 ±0.67
b
37.33 ±0.93
b
42.67 ±1.17
a
Note: Values with different superscripts in each row are significantly different (P > 0.05)
The mean final serum blood glucose values ranged from 45.67 ±1.45 g/100 ml to 51.33 ±1.20 g/100 ml. All the
final mean blood glucose values were higher than the initial value (35.67 ±0.88 g/100 ml). The highest value was
from the fish fed AMX2 diet and the lowest value was from fish fed AMX3 diet. The fish fed the AMX2, AMX1
had significantly higher (p < 0.05) mean blood glucose than AMX3 diets (Table 4).
The mean final aspartate aminotransferase (AST) values ranged from 18.33 ±3.38 to 29.67 ±3.18 µ/l. All the
mean final blood AST values were higher compared to the initial value 20.00 ±2.31 µ/l except AMX1 diet. There
were no significant differences between the highest and lowest final blood AST values of all the fish fed AMX’s
diets (Table 4). The mean final alanine aminotransferase (ALT) values ranged from 61.00 ± 2.08 to 87.33 ±
1.76µ/l. All the fish fed AMX’s experimental diets were higher in ALT compared to the initial value of 72.00 ±
6.43 µ/l except AMX1. The highest value was from fish fed AMX3 diet while the lowest was from AMX1 diet.
The fish fed the AMX3 and AMX2 had significantly higher (p < 0.05) mean ALT than AMX1 diet. The blood
final mean ALP ranged from 35.33 ±0.67 to 42.67 ±1.17 µ/l. The highest value was from AMX3 diet, while the
lowest was from the fish fed AMX1 diet. Fish fed AMX3 diet had significantly higher (p < 0.05) ALP than the
fish fed AMX2 and AMX1 diets (Table 4).
