International Journal of Aquaculture, 2016, Vol.6, No.15, 1
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from fish fed AMX2 diet and the lowest value of 0.52 ±0.02% / day was from the fish fed control diet. The fish
fed the control diet had significantly lower (p < 0.05) SGR compared to others. There was no significant
difference (p < 0.05) in the SGR between the fish fed AMX2 and AMX3 diets (Table 2). The RGR of the fish fed
the experimental diets followed the same trends as SGR.
The final condition factor (K2) had the highest value of 0.48 ±0.03 from fish fed AMX3 diet and lowest value of
0.39 ± 0.09 from fish fed AMX2 diet. There was no significant difference in the K2 of all the fish fed the
experimental diets (Table 2). The percentage survival of the fish fed the tested diets significantly (P < 0.05)
ranged from 80.00 ±0.00 to 90.00 ±0.00%. The highest value was from the fish fed AMX2 diet and lowest value
of 80.00 ±0.00% was from fish fed the control diet.
The total feed intake (TFI) ranged from 1701.18 ±27.69 – 2362.95 ±64.19 g. The TFI was significantly different
among the treated diets, with the highest value of 2362.19g from the fish fed AMX3 and the least 1701.18 ±27.69
g from the fish fed AMX1 (Control). The mean feed intake (MFI), total protein intake (TPI) and mean protein
intake (MPI) also varied significantly within the treatment and followed the same trend as the TPI in the
experiment. The feed conversion ratio (FCR) followed the opposite trend of the TFI, MFI and MPI. The highest
FCR was from the fish fed the control, while the lowest was from fish fed the AMX2 diets. The protein efficiency
ratio (PER) was significantly different among the treatments. The highest value of 0.87 ±0.00 was from the fish
fed AMX2 and the least value of 0.54 ±0.01 was from the fish fed AMX1 diet (Table 2).
The pack cell volume (PCV) values ranged from 22.33 ±0.88 to 27.67 ±1.45%. The highest value was from the
fish fed AMX2 diet, followed closely by 26.67 ±1.89% from AMX3 diet and the lowest value was from the
control diet. All the final PCV values were higher than the initial (Table 3). The fish fed AMX2, had significantly
(P < 0.05) higher PCV than AMX1 (control) diet but differed insignificantly. (P > 0.05) from fish fed AMX3 diet
(Table 3). The red blood cell hemoglobin (Hb) from the fish fed all experimental diets followed the same pattern
as PCV. The red blood cell (RBC) counts ranged from 11.64 ±1.49 to 17.47 ±1. 57 x 10
6
/dl. The highest RBC
counts value was from the fish fed AMX3 diet, followed closely by 14.57 ± 0.58 from AMX2 diet, while the
lowest value was from the fish fed control diet (AMX1). All the final RBC counts were higher than the initial, the
fish fed AMX3 diets had significantly (P < 0.05) higher RBC counts than the control (AMX1), but differed
insignificantly (p > 0.05) from the fish fed AMX2 diets (Table 3). The mean cell volume (MCH) parameter of the
red blood cell ranged from 63.00 ±3.00 to 65.67 ±2.85 x10
4
/l. The highest value was from the fish fed AMX1
diet, followed closely by 65.33 ±1.45 x10
4
/l from the fish fed AMX2 diet, while the lowest value was from the
fish fed AMX3 diet. All the final MCH were higher than the initial value of 55.00 ±7.00 x10
4
/l. There was no
significant difference (P > 0.05) in the MCV between the fish fed the control diet and other diets (Table 3). All the
final MCH and MCHC values were higher than the initial values except the fish fed AMX3 diet. There were no
significant difference (P > 0.05) in the MCH and MCHC values between the fish fed the control diet and fish fed
the other diets (Table 3).
The white blood cell (WBC) counts ranged from 8.87 ±1.45 to 11.82 ±0.70 x10
3
/dl. The highest value was from
the fish fed control diet, followed closely by 9.20 ±0.35 x10
3
/dl from fish fed AMX2 diet, while the lowest was
from the fish fed AMX3 diet. All the final WBC counts were higher (P < 0.05) than the initial. The fish fed the
control diet, had significantly higher (P < 0.05) WBC counts than others (Table 3).The erythrocyte sedimentation
rate (ESR) varied from 2.00 ±1.00 to 2.67 ±0.67 mm/hr. The fish fed AMX3 had the highest value for ESR,
while the lowest value was from the fish fed AMX2 diet. The final ESR was higher than the initial and there was
not significantly difference (P > 0.05) among the ESR value of the fish fed the experimental diets (Table 3).
The mean final total protein for the fish fed the experimental diets ranged from 6.30 ±0.15 g/100 ml to 7.27 ±
0.09 g/100 ml. All the final total proteins levels were higher than the initial value of 5.83 ±0.007 g/100 ml.The
highest total serum proteins was from the fish fed AMX3 diet and the lowest value was from the fish fed AMX1
diet. The fish fed the tested diets had significantly (p<0.05). (p<0.05) higher mean final total protein than the fish
