基本HTML版本
Computational Molecular Biology 2015, Vol. 5, No. 1, 1-13
http://cmb.biopublisher.ca
10
O- glycosylation
39
T
Non-glycosylated
40
T
Non-glycosylated
42
T
Potential glycosylated
50
S
Non-glycosylated
53
S
Non-glycosylated
68
T
Non-glycosylated
Protein
Glycosylation Type
Position
Sequence
Prediction
HBB
Camelus bactrianus
,
dromedarius
and
ferus
N- glycosylation
10
N
AV
Non-glycosylated
57
N
NP
Non-glycosylated
58
N
PK
Non-glycosylated
70
N
SF
Non-glycosylated
77
N
HL
Non-glycosylated
81
N
LK
Non-glycosylated
103
N
FR
Non-glycosylated
109
N
VL
Non-glycosylated
140
N
AL
Non-glycosylated
O- glycosylation
5
S
Non-glycosylated
17
S
Non-glycosylated
39
T
Non-glycosylated
45
S
Non-glycosylated
50
S
Non-glycosylated
51
T
Non-glycosylated
66
S
Non-glycosylated
71
S
Non-glycosylated
85
T
Non-glycosylated
90
S
Non-glycosylated
124
T
Non-glycosylated
HBB
Homo sapiens
N- glycosylation
20
N
VD
Non-glycosylated
58
N
PK
Non-glycosylated
81
N
LK
Non-glycosylated
103
N
FR
Non-glycosylated
109
N
VL
Non-glycosylated
140
N
AL
Non-glycosylated
O- glycosylation
5
T
Potential glycosylated
10
S
Non-glycosylated
13
T
Non-glycosylated
39
T
Non-glycosylated
45
S
Non-glycosylated
50
S
Non-glycosylated
51
T
Non-glycosylated
73
S
Non-glycosylated
85
T
Non-glycosylated
88
T
Non-glycosylated
90
S
Non-glycosylated
124
T
Non-glycosylated
we found potential glycosylated only for Thr5 in
Homo sapiens
(Table 4). No position is susceptible to
O-glycosylation in camel HBB. O-glycosylation
occurs by the addition of glucose to free hydroxyl
group containing AAs residues that includes Ser, Thr
and to some extent, hydroxylproline and
hydroxylysine (Christlet et al., 2001). Therefore we
conclude that O-linked glycosylation occurs most than
N-,C-linked glycosylation in HBA, and but HBB is
resistant to N,O-glycosylation specially in camels
however alpha hemoglobin is most susceptible to
O-linked glycosylation, maybe it depends on alcoholic
factor in camel and human amino acids.
2.7 Analysis of PSI-BLAST (Position-Specific
Iterated BLAST)