Bioscience Methods 2015, Vol.6, No.3, 1-9
4
Figure 1 Compatibility of
Trichoderma
spp with Mancozeb &
Captan fungicides.
Note: a) 1-
T. viride
(Control), 2-Mancozeb 8000 μg/ml, 3-Captan 700
μg/ml; b) 1-
T. harzianum
(Control), 2-Mancozeb 8000 μg/ml, 3-Captan
700 μg/ml; c) 1-
T. koningii
(Control), 2-Mancozeb 8000 μg/ml,
3-Captan 600 μg/ml; d) 1-
T. pseudokoningii
(Control), 2-Mancozeb
5000 μg/ml, 3-Captan 500 μg/ml; e) 1-
T. virens
(Control), 2-Mancozeb
8000 μg/ml , 3-Captan 500 μg/ml
was very low in concentration of 7000 μg/ml, no
growth occurred when Mancozeb was used above
the @8000 μg/ml.
T. koningii
showed growth at
Captan @100, 200, 300 μg/ml. The growth was
slow at Captan @ 500 and 600μg/ml. No growth
was observed on Captan@700μg/ml.
d.
Trichoderma pseudokoningii
:
Trichoderma pseudokoningii
grow slow under media containing Mancozeb@1000 2000,
3000 and 4000 μg/ml, but it was inhibited completely at
Mancozeb@5000μg/ml. The radial growth of
T.
pseudokoningii
was also low at Captan@100, 200, 300 and
400μg/ml. But no growth was observed on Captan@500
μg/ml.
e.
Trichoderma virens
:
Trichoderma virens
grow readily
under low concentration of Mancozeb(@1000 – 5000
μg/ml). The radial growth was gradually decreased at
Mancozeb@5000 to 7000 μg/ml and no growth
was recorded at 8000 μg/ml. Treatment of Captan
Figure 2Antagonistic activity of
Trichoderma
spp on pathogenic fungi.
Note: Row 1:
Alternaria alternata
(a-control, b-
T. virens
, c-
T. koningii
)
Row2:
Rhizoctonia solani
(a-control, b-
T. koningii
, c-
T. hrzianum
)
Row3:
Aspergillus niger
(a-control, b-
T. koningii
, c-
T.viride
)
Row4:
Geotrichum candidum
(a-control, b-
T. virens
, c-
T.koningii
)
Row 5:
Fusarium oxysporum
f. sp.spinaciae (a-control, b-
T. virens
,
c-
T.viride
)
Row6:
Macrophomina phaseolina
(a-control, b-
T. viride
, c-
T. virens
)
@100 to 300 μg/ml, the radial growth of
T. virens
was recorded. However, growth was stopped on
Captan @ 500 μg/ml.
Only,
T. pseudokoningii
was found susceptible at
lower tested concentrations on both fungicides. The
obtained results indicated that low concentration of
Mancozeb and Captan does not affect the radial
growth of mycelium of
Trichoderma
spp. However,
increased concentration of fungicides were decreased
the radial growth of mycelium.
3.2 Antagonistic activity of
Trichoderma
species
against pathogenic fungi
Isolated plant pathogens i.e.
Alternaria alternata,
Rhizoctonia solani, Aspergillus niger, Geotrichum
candidum, Fusarium oxysporum
f. sp.
spinacae
and
Macrophomina phaseolina
were evaluated their
antagonistic nature against
Trichoderma
species under
